Sortase anchored proteins of Streptococcus uberis play major roles in the pathogenesis of bovine mastitis in dairy cattle.
Sortase anchored proteins of Streptococcus uberis play major roles in the pathogenesis of bovine mastitis in dairy cattle.
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DOI：
10.1051/vetres/2010036
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发表时间：
2010-09
影响因子：
4.4
通讯作者：
Coffey TJ
中科院分区：
文献类型：
--
作者：
Leigh JA;Egan SA;Ward PN;Field TR;Coffey TJ
链球菌菌株0140J含有单拷贝分选酶A（srtA），其编码能够将特异性蛋白共价锚定至肽聚糖的转酰胺酶。与野生型不同的是，携带一个失活的ISS1插入srtA内的同基因突变体只能感染牛乳腺在一个短暂的方式。对于攻击后的第一个24小时，srtA突变体以与野生型菌株相似的速率和数量定殖，但与野生型不同，srtA突变体随后没有以更高的数量定殖。在对两种菌株感染的反应中检测到相似水平的宿主细胞浸润，但仅在野生型菌株感染的那些乳房区中，疾病的临床体征明显。分离不能表达单个分选酶底物蛋白（sub0135、sub0145、sub0207、sub0241、sub0826、sub0888、sub1095、sub1154、sub1370和sub1730）的突变体，并在相同的攻击模型中测定其毒力。这表明缺乏sub0145、sub1095和sub1154的突变体在牛中是减毒的。这些数据表明，许多分选酶锚定蛋白在S.泌乳牛乳腺内的支原体感染。
Streptococcus uberis, strain 0140J, contains a single copy sortase A (srtA), encoding a transamidase capable of covalently anchoring specific proteins to peptidoglycan. Unlike the wild-type, an isogenic mutant carrying an inactivating ISS1 insertion within srtA was only able to infect the bovine mammary gland in a transient fashion. For the first 24 h post challenge, the srtA mutant colonised at a similar rate and number to the wild type strain, but unlike the wild type did not subsequently colonise in higher numbers. Similar levels of host cell infiltration were detected in response to infection with both strains, but only in those mammary quarters infected with the wild type strain were clinical signs of disease evident. Mutants that failed to express individual sortase substrate proteins (sub0135, sub0145, sub0207, sub0241, sub0826, sub0888, sub1095, sub1154, sub1370, and sub1730) were isolated and their virulence determined in the same challenge model. This revealed that mutants lacking sub0145, sub1095 and sub1154 were attenuated in cattle. These data demonstrate that a number of sortase anchored proteins each play a distinct, non-redundant and important role in pathogenesis of S. uberis infection within the lactating bovine mammary gland.
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影响因子：
3.1
作者：
CLEARY, PP;PRAHBU, U;HANDLEY, J
通讯作者：
HANDLEY, J
影响因子：
3.1
作者：
Janulczyk, R;Rasmussen, M
通讯作者：
Rasmussen, M
影响因子：
4.4
作者：
Bannerman, DD;Paape, MJ;Hope, JC
通讯作者：
Hope, JC
影响因子：
3.5
作者：
Hoeben, D;Burvenich, C;Hard, DL
通讯作者：
Hard, DL
DOI：
10.1111/j.1574-695x.1994.tb00432.x
发表时间：
1994-02-01
影响因子：
--
作者：
HILL, AW;FINCH, JM;LEIGH, JA
通讯作者：
LEIGH, JA