Large-scale transcriptome comparison reveals distinct gene activations in wheat responding to stripe rust and powdery mildew.
Large-scale transcriptome comparison reveals distinct gene activations in wheat responding to stripe rust and powdery mildew.
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大规模转录组比较揭示了小麦响应条锈病和白粉病的不同基因激活
DOI：
10.1186/1471-2164-15-898
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发表时间：
2014-10-15
期刊：
影响因子：
4.4
通讯作者：
Ji W
中科院分区：
文献类型：
--
作者：
Zhang H;Yang Y;Wang C;Liu M;Li H;Fu Y;Wang Y;Nie Y;Liu X;Ji W
条锈病(Puccinia striiformisf.)Sp.tritici；Pst)和白粉病(Blumeria graminisf.小麦(Tritici；BGT)是小麦(Triticum Aestivum)的重要病害。由于这两种病原菌都是生物营养真菌，因此推测参与寄主防御反应的机制和基因转录本相似。本研究的主要目的是鉴定接种PstorBgt后表达模式发生变化的共调控mRNAs，并鉴定真菌胁迫反应的特异性mRNAs。结果在接种中国小麦Pstrace Cyr 31的六倍体小麦品系N9134与接种Bgtrace E09的六倍体小麦品系N9134在接种后1、2和3d的转录组进行了比较。PStandBg感染可影响23.8%的ALT细胞的转录。再生障碍物基因。与对Pst感染的反应相比，BGT感染在N9134细胞中引起了更强烈的基因表达变化。一系列具有不同表达模式的重叠基因簇提供了对Bgt和Pst感染反应的调控差异的洞察。差异表达基因被分成7条富含Bgt侵染叶片的京都百科全书和4条侵染Pst叶片的基因和基因组途径，但只有2条途径重叠。在植物-病原菌相互作用途径中，N9134对Bgt侵染的反应比对Pst入侵的反应激活了更多的基因和途径。基因组分析表明，小麦基因组中有一些共同的微生物基因片段，这些片段是Bgt和Pst侵染后特异诱导的。结论综合考虑，小麦N9134对Bgt和Pst侵染的反应途径和激活的基因存在差异。本研究获得的小麦-真菌相互作用的海量序列数据为今后小麦-真菌相互作用的功能和分子研究提供了有力的平台。
BackgroundStripe rust (Puccinia striiformisf. sp.tritici;Pst) and powdery mildew (Blumeria graminisf. sp.tritici;Bgt) are important diseases of wheat (Triticum aestivum) worldwide. Similar mechanisms and gene transcripts are assumed to be involved in the host defense response because both pathogens are biotrophic fungi. The main objective of our study was to identify co-regulated mRNAs that show a change in expression pattern after inoculation withPstorBgt, and to identify mRNAs specific to the fungal stress response.ResultsThe transcriptome of the hexaploid wheat line N9134 inoculated with the ChinesePstrace CYR 31 was compared with that of the same line inoculated withBgtrace E09 at 1, 2, and 3 days post-inoculation. Infection byPstandBgtaffected transcription of 23.8% of allT. aestivumgenes. Infection byBgttriggered a more robust alteration in gene expression in N9134 compared with the response toPstinfection. An array of overlapping gene clusters with distinctive expression patterns provided insight into the regulatory differences in the responses toBgtandPstinfection. The differentially expressed genes were grouped into seven enriched Kyoto Encyclopedia of Genes and Genomes pathways inBgt-infected leaves and four pathways inPst-infected leaves, while only two pathways overlapped. In the plant–pathogen interaction pathway, N9134 activated a higher number of genes and pathways in response toBgtinfection than in response toPstinvasion. Genomic analysis revealed that the wheat genome shared some microbial genetic fragments, which were specifically induced in response toBgtandPstinfection.ConclusionsTaken together, our findings indicate that the responses of wheat N9134 to infection byBgtandPstshows differences in the pathways and genes activated. The mass sequence data for wheat–fungus interaction generated in this study provides a powerful platform for future functional and molecular research on wheat–fungus interactions.
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影响因子：
20.5
作者：
Boudsocq, Marie;Sheen, Jen
通讯作者：
Sheen, Jen
影响因子：
4.4
作者：
Kroupitski, Yulia;Golberg, Dana;Sela, Shlomo
通讯作者：
Sela, Shlomo
DOI：
10.1073/pnas.0606330103
发表时间：
2006-11-14
影响因子：
11.1
作者：
Fauteux, Francois;Chain, Florian;Belanger, Richard R.
通讯作者：
Belanger, Richard R.
影响因子：
6.9
作者：
Bhuiyan NH;Selvaraj G;Wei Y;King J
通讯作者：
King J
影响因子：
3.5
作者：
Jurkowski, GI;Smith, RK;Bent, AF
通讯作者：
Bent, AF