Effects of Dietary Nano-Zinc Oxide Supplementation on Meat Quality, Antioxidant Capacity and Cecal Microbiota of Intrauterine Growth Retardation Finishing Pigs.
Effects of Dietary Nano-Zinc Oxide Supplementation on Meat Quality, Antioxidant Capacity and Cecal Microbiota of Intrauterine Growth Retardation Finishing Pigs.
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DOI：
10.3390/foods12091885
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发表时间：
2023-05-04
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影响因子：
--
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中科院分区：
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--
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纳米氧化锌（Nano-ZnO）是一种新型的纳米材料，具有抗氧化和肠道保护作用，因此，我们假设日粮中添加纳米氧化锌可以调节自然发生的宫内生长迟缓（IUGR）育肥猪模型的不良肉质、氧化应激和肠道菌群失调。根据出生体重，共选择6头正常出生体重（NBW）和12头IUGR仔猪。NBW组饲喂基础日粮，IUGR组随机分为2组，分别饲喂基础日粮和添加600 mg/kg纳米ZnO的日粮。纳米ZnO通过降低48 h滴水损失、蒸煮损失、剪切力和MyHc IIx mRNA表达，提高红度（a*）、固定水峰面积比（P22）、肌节长度和MyHc Ia mRNA表达，改善IUGR引起的肉质下降。纳米ZnO通过促进核因子红细胞2相关因子2-谷氨酰半胱氨酸连接酶（Nrf 2-GCL）的核转位，激活Nrf 2-GCL信号通路，提高GCL活性及其催化/修饰亚基（GCLC/GCLM）的mRNA和蛋白表达，从而减轻IUGR相关的肌肉氧化损伤。此外，纳米ZnO改变了IUGR猪盲肠微生物群的组成，如Shannon和Simpson指数的变化，增强的UCG-005，hoa 5 - 07 d 05肠道群和Rikenellaceae RC 9肠道群丰度所示。UCG-005和hoa 5 - 07 d 05肠群丰度与反映肉质性状和抗氧化特性的指标相关。总之，纳米ZnO通过改变肌肉的持水性、水分分布和超微结构，激活Nrf 2-GCL信号通路以缓解氧化状态，调节盲肠微生物组成，改善IUGR损伤的肉质。
As nano-zinc oxide (Nano-ZnO), a new type of nanomaterial, has antioxidant and intestinal protection effects, we hypothesized that dietary Nano-ZnO could modulate poor meat quality, oxidative stress and disturbed gut microbiota in the finishing pig model of naturally occurring intrauterine growth retardation (IUGR). A total of 6 normal-born weight (NBW) and 12 IUGR piglets were selected based on birth weight. The pigs in the NBW group received a basal diet, and IUGR pigs were randomly divided into two groups and treated with basal diet and 600 mg/kg Nano-ZnO-supplemented diet. Dietary Nano-ZnO ameliorated IUGR-associated declined meat quality by lowering the drip loss48h, cooking loss, shearing force and MyHc IIx mRNA expression, and raising the redness (a*), peak area ratio of immobilized water (P22), sarcomere length and MyHc Ia mRNA expression. Nano-ZnO activated the nuclear factor erythroid 2-related factor 2-glutamyl cysteine ligase (Nrf2-GCL) signaling pathway by promoting the nuclear translocation of Nrf2, increasing the GCL activities, and mRNA and protein expression of its catalytic/modify subunit (GCLC/GCLM), thereby attenuating the IUGR-associated muscle oxidative injury. Additionally, the composition of IUGR pigs’ cecal microbiota was altered by Nano-ZnO, as seen by changes in Shannon and Simpson indexes, the enhanced UCG-005, hoa5-07d05 gut group and Rikenellaceae RC9 gut group abundance. The UCG-005 and hoa5-07d05 gut group abundance were correlated with indicators that reflected the meat quality traits and antioxidant properties. In conclusion, Nano-ZnO improved the IUGR-impaired meat quality by altering water holding capacity, water distribution and the ultrastructure of muscle, activating the Nrf2-GCL signaling pathway to alleviate oxidative status and regulating the cecal microbial composition.
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影响因子：
7
作者：
Chen Y;Zhang H;Chen Y;Jia P;Ji S;Zhang Y;Wang T
通讯作者：
Wang T
影响因子：
4.4
作者：
Ha, Khoi-Nguyen;Chen, Yan;Sternberg, Paul, Jr.
通讯作者：
Sternberg, Paul, Jr.
影响因子：
48
作者：
Callahan BJ;McMurdie PJ;Rosen MJ;Han AW;Johnson AJ;Holmes SP
通讯作者：
Holmes SP
影响因子：
2
作者：
Chen, Fang;Zhang, Hao;Wei, Jintao
通讯作者：
Wei, Jintao
影响因子：
7.1
作者：
Joo, S. T.;Kim, G. D.;Ryu, Y. C.
通讯作者：
Ryu, Y. C.