Quantitative and antioxidative behavior of Trolox in rats' blood and brain by HPLC-UV and SMFIA-CL methods.
Quantitative and antioxidative behavior of Trolox in rats' blood and brain by HPLC-UV and SMFIA-CL methods.
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通过 HPLC-UV 和 SMFIA-CL 方法测定大鼠血液和脑中 Trolox 的定量和抗氧化行为。
DOI：
10.1002/bio.2975
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发表时间：
2016
期刊：
影响因子：
2.9
通讯作者：
Nakashima K
中科院分区：
文献类型：
--
作者：
Wada M;Wada M;Ikeda R;Fuchigami Y;Koyama H;Ohkawara S;Kawakami S;Kuroda N;Nakashima K
Trolox是一种水溶性维生素E类似物，由于其高抗氧化作用，已在Trolox等效抗氧化能力和氧自由基抗氧化能力试验中用作阳性对照。本研究采用新建立的半微量流动注射分析法、化学发光检测法和HPLC-UV法评价了Trolox的体外抗氧化作用及其在大鼠血液和脑微透析液中的浓度。在给药试验中，可以监测大鼠单次给予200 mg/kg Trolox后，Trolox在血液和脑微透析液中的抗氧化作用。血和脑的抗氧化作用分别为12.0 ± 2.1和8.4 ± 2.1 × 103抗氧化作用% × min。此外，使用定量数据计算的血液和脑的曲线下面积（AUC）s 0 -360（n= 3）分别为10.5 ± 1.2和9.7 ± 2.5 mg/mL × min。该结果表明Trolox通过血脑屏障的可转移性高。由于浓度和抗氧化作用之间具有良好的相关性（r≥ 0.702），因此可以证实Trolox在血液和脑中引起的抗氧化作用增加。阐明了Trolox在大鼠脑内具有抗氧化作用的事实。版权所有© 2015约翰威利父子有限公司.
Trolox, a water‐soluble vitamin E analogue has been used as a positive control in Trolox equivalent antioxidant capacity and oxygen radical antioxidant capacity assays due to its high antioxidative effect. In this study, theex vivoantioxidative effects of Trolox and its concentration in blood and brain microdialysates from rat after administration were evaluated by newly established semi‐microflow injection analysis, chemiluminescence detection and HPLC‐UV. In the administration test, the antioxidative effect of Trolox in blood and brain microdialysates after a single administration of 200 mg/kg of Trolox to rats could be monitored. The antioxidative effects in blood (12.0 ± 2.1) and brain (8.4 ± 2.1, × 103antioxidative effect % × min) also increased. Additionally, the areas under the curve (AUC)s0–360(n= 3) for blood and brain calculated with quantitative data were 10.5 ± 1.2 and 9.7 ± 2.5 mg/mL × min, respectively. This result indicates that Trolox transferability through the blood–brain barrier is high. The increase in the antioxidative effects caused by Trolox in the blood and brain could be confirmed because good correlations between concentration and antioxidative effects (r≥ 0.702) were obtained. The fact that Trolox can produce an antioxidative effect in rat brain was clarified. Copyright © 2015 John Wiley & Sons, Ltd.
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