Toward a Systemic Understanding of Listeria monocytogenes Metabolism during Infection.
Toward a Systemic Understanding of Listeria monocytogenes Metabolism during Infection.
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DOI：
10.3389/fmicb.2012.00023
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发表时间：
2012
影响因子：
5.2
通讯作者：
Dandekar T
中科院分区：
文献类型：
--
作者：
Fuchs TM;Eisenreich W;Kern T;Dandekar T
单核细胞增生李斯特菌是一种食源性人类病原体，可引起易感动物和人类的侵袭性感染。对于宿主内的增殖，这种兼性细胞内病原体使用特定代谢途径、转运蛋白和酶功能的储库，其表达需要复杂调控网络的协调活性。L.的高度适应代谢。单核细胞增多症的发生强烈依赖于感染期间遇到的各种环境的营养成分。转录组学和蛋白质组学研究揭示了该病原体在培养细胞内或体内复制过程中基因表达的时空动态。代谢线索是利用不寻常的C2和C3体，丙酮酸的代谢，硫胺素的可用性，肽的摄取，某些氨基酸的获得或生物合成，以及通过戊糖磷酸途径降解葡萄糖磷酸。这些实施例说明了体内条件对能量、碳和氮代谢的干扰，从而影响植物生长。利用，分析和建模的可用数据集作为第一次尝试系统地了解感染过程中的代谢。L.单核细胞增多症可以作为革兰氏阳性兼性细胞内细菌的系统生物学的模式生物。
Listeria monocytogenes is a foodborne human pathogen that can cause invasive infection in susceptible animals and humans. For proliferation within hosts, this facultative intracellular pathogen uses a reservoir of specific metabolic pathways, transporter, and enzymatic functions whose expression requires the coordinated activity of a complex regulatory network. The highly adapted metabolism of L. monocytogenes strongly depends on the nutrient composition of various milieus encountered during infection. Transcriptomic and proteomic studies revealed the spatial–temporal dynamic of gene expression of this pathogen during replication within cultured cells or in vivo. Metabolic clues are the utilization of unusual C2- and C3-bodies, the metabolism of pyruvate, thiamine availability, the uptake of peptides, the acquisition or biosynthesis of certain amino acids, and the degradation of glucose-phosphate via the pentose phosphate pathway. These examples illustrate the interference of in vivo conditions with energy, carbon, and nitrogen metabolism, thus affecting listerial growth. The exploitation, analysis, and modeling of the available data sets served as a first attempt to a systemic understanding of listerial metabolism during infection. L. monocytogenes might serve as a model organism for systems biology of a Gram-positive, facultative intracellular bacterium.
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影响因子：
3.3
作者：
Donaldson, Janet R.;Nanduri, Bindu;Lawrence, Mark L.
通讯作者：
Lawrence, Mark L.
影响因子：
4.4
作者：
Dumas, Emilie;Meunier, Bruno;Hebraud, Michel
通讯作者：
Hebraud, Michel
影响因子：
3.1
作者：
ALEXANDER, JE;ANDREW, PW;ROBERTS, IS
通讯作者：
ROBERTS, IS
影响因子：
3.1
作者：
Chatterjee, SS;Hossain, H;Hain, T
通讯作者：
Hain, T
影响因子：
64.8
作者：
BIELECKI, J;YOUNGMAN, P;PORTNOY, DA
通讯作者：
PORTNOY, DA