Overexpression of VqWRKY31 enhances powdery mildew resistance in grapevine by promoting salicylic acid signaling and specific metabolite synthesis.
Overexpression of VqWRKY31 enhances powdery mildew resistance in grapevine by promoting salicylic acid signaling and specific metabolite synthesis.
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VqWRKY31 的过表达通过促进水杨酸信号传导和特定代谢物合成来增强葡萄树的白粉病抗性
DOI：
10.1093/hr/uhab064
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发表时间：
2022-01-19
影响因子：
8.7
通讯作者：
Wang X
中科院分区：
文献类型：
--
作者：
Yin W;Wang X;Liu H;Wang Y;Nocker S;Tu M;Fang J;Guo J;Li Z;Wang X
Abstract Powdery mildew (PM), caused by the fungal pathogen Erysiphe necator, is one of the most destructive diseases of grapevine (Vitis vinifera and other Vitis spp.). Resistance to PM is an important goal for cultivar improvement, and understanding the underlying molecular mechanisms conditioning resistance is critical. Here, we report that transgenic expression of the WRKY transcription factor gene VqWRKY31 from the PM-resistant species Vitis quinquangularis conferred resistance to PM in V. vinifera through promoting salicylic acid signaling and specific metabolite synthesis. VqWRKY31 belongs to the WRKY IIb subfamily, and expression of the VqWRKY31 gene was induced in response to E. necator inoculation. Transgenic V. vinifera plants expressing VqWRKY31 were substantially less susceptible to E. necator infection, and this was associated with increased levels of salicylic acid and reactive oxygen species. Correlation analysis of transcriptomic and metabolomic data revealed that VqWRKY31 promoted expression of genes in metabolic pathways and the accumulation of many disease resistance-related metabolites, including stilbenes, flavonoids, and proanthocyanidins. In addition, results indicated that VqWRKY31 can directly bind to the promoters of two structural genes in stilbene synthesis, STS9 and STS48, and activate their expression. Based on our results, we propose a model where VqWRKY31 enhances grapevine PM resistance through activation of salicylic acid defense signaling and promotion of specific disease resistance-related metabolite synthesis. These findings can be directly exploited for molecular breeding strategies to produce PM-resistant grapevine germplasm.
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影响因子：
5.6
作者：
Dai L;Wang D;Xie X;Zhang C;Wang X;Xu Y;Wang Y;Zhang J
通讯作者：
Zhang J
影响因子：
7.2
作者：
Bhattarai, Kishor K.;Atamian, Hagop S.;Eulgem, Thomas
通讯作者：
Eulgem, Thomas
一种用于大规模检测、鉴定和定量广泛目标代谢物的新型综合方法：在水稻代谢组学研究中的应用。
影响因子：
27.5
作者：
Chen, Wei;Gong, Liang;Luo, Jie
通讯作者：
Luo, Jie
影响因子：
3.8
作者：
Hammerbacher, Almuth;Raguschke, Bettina;Gershenzon, Jonathan
通讯作者：
Gershenzon, Jonathan
影响因子：
3.7
作者：
Chang X;Heene E;Qiao F;Nick P
通讯作者：
Nick P