Molecular Cloning, Characterization, and Expression Analysis of SIMILAR TO RCD-ONE (SRO) Family Genes Responding to Abiotic and Biotic Stress in Cucumber
Molecular Cloning, Characterization, and Expression Analysis of SIMILAR TO RCD-ONE (SRO) Family Genes Responding to Abiotic and Biotic Stress in Cucumber
复制标题
黄瓜响应非生物和生物胁迫的类似 RCD-ONE (SRO) 家族基因的分子克隆、表征和表达分析
DOI：
10.3390/horticulturae8070634
复制
发表时间：
2022-07
期刊：
影响因子：
3.1
通讯作者：
Yong Zhou
中科院分区：
文献类型：
--
作者：
Lingdi Xiao;Zixian Zhou;Chuxia Zhu;Jindong Zhao;Zhaoyang Hu;Shiqiang Liu;Yong Zhou
与RCD-ONE类似，SRO是一个植物特异性的小蛋白家族，它控制着许多生物过程，包括生理发育和逆境反应。SRO基因家族已经在许多植物物种中被研究过，但在黄瓜中还没有详细的表征和表达谱。在这项研究中，我们对黄瓜的SRO基因进行了表征，并测定了其在不同组织和暴露于不同生物和非生物胁迫下的转录水平。鉴定并分离到4个SRO基因，命名为CsSRO1-CsSRO4，分布在3条不同的染色体上。基因重复分析表明，只鉴定出一对片段重复事件，未检测到串联重复事件。所有cssro均由PARP结构域和c端RST结构域组成，而n端WWE结构域仅在CsSRO2和CsSRO4中存在。根据系统发育树，将15种植物的sro分为I和II两类，I类又可分为Ia ~ Id 4个亚群。保守基序和基因结构分析表明，在系统发育树的同一分支中的sro具有相似的保守基序配置和基因结构。然而，SRO基因在不同亚群中具有不同数量的内含子，这可能会影响新家族成员的进化。RNA-Seq数据和qRT-PCR结果显示，4个CsSRO基因在不同组织和不同胁迫下具有不同的表达模式，提示它们在植物生长和胁迫应答中具有多种功能。研究结果为进一步研究CsSRO基因在黄瓜发育和胁迫相关过程中的功能调控机制奠定了基础。
SIMILAR TO RCD-ONE (SRO) is a plant-specific small protein family that controls many biological processes including physiological development and stress responses. The SRO gene family has been studied in several plant species, but no detailed characterization and expression profiles of this important gene family were performed in cucumber. In this study, we characterize the SRO genes in cucumber, and determined their transcript levels in various tissues and under exposure to diverse biotic and abiotic stressors. Four SRO genes (named as CsSRO1–CsSRO4) were identified and isolated, which were distributed on three different chromosomes. Gene duplication analysis showed that only one pair of segmental duplication event was identified, but no tandem duplication events were detected. All CsSROs consist of the PARP domain and a C-terminal RST domain, while the N-terminal WWE domain was only present in CsSRO2 and CsSRO4. SROs from 15 plant species are divided into two groups (I and II), and group I can be further divided into four subgroups (Ia to Id) according to the phylogenetic tree. The conserved motif and gene structure analyses showed that SROs within the same branch of the phylogenetic tree have analogous conserved motifs configuration and gene structures. However, SRO genes possessed variable numbers of introns in different subgroups, which may affect the evolution of new family members. RNA-Seq data and qRT-PCR results showed that the four CsSRO genes have distinct expression pattern in various tissues and under diverse stresses, suggesting their multiple functions in plant growth and stress responses. The findings provide a basis for further research aiming at functional characterization of the regulatory mechanism to reveal the roles of CsSRO genes in developmental and stress-related processes of cucumber.
登录
查看更多内容
影响因子：
5.6
作者：
Liu A;Wei M;Zhou Y;Li D;Zhou R;Zhang Y;Zhang X;Wang L;You J
通讯作者：
You J
影响因子：
3.7
作者：
Li N;Xu R;Wang B;Wang J;Huang S;Yu Q;Gao J
通讯作者：
Gao J
影响因子：
6.5
作者：
Jiang, Wenqiang;Geng, Yuepan;Yin, Junliang
通讯作者：
Yin, Junliang
DOI：
10.1073/pnas.0604711103
发表时间：
2006-12-05
影响因子：
11.1
作者：
Katiyar-Agarwal, Surekha;Zhu, Jianhua;Zhu, Jian-Kang
通讯作者：
Zhu, Jian-Kang
影响因子：
2.9
作者：
Xiaoliang Zhao;Lijie Gao;Jiayan Ren;Fei Pan
通讯作者：
Xiaoliang Zhao;Lijie Gao;Jiayan Ren;Fei Pan