Genome-Wide Identification and Evolutionary Analysis of the SRO Gene Family in Tomato.
Genome-Wide Identification and Evolutionary Analysis of the SRO Gene Family in Tomato.
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DOI：
10.3389/fgene.2021.753638
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发表时间：
2021
影响因子：
3.7
通讯作者：
Gao J
中科院分区：
文献类型：
--
作者：
Li N;Xu R;Wang B;Wang J;Huang S;Yu Q;Gao J
SRO（类似于RCD ONE）是一类植物特异性小分子蛋白，在植物生长发育和环境响应中发挥着重要作用。然而，番茄中的 SRO 仍然缺乏系统的表征。基于生物信息学方法，从栽培番茄和几种野生番茄中鉴定和表征了SRO家族基因。采用qRT-PCR研究SRO基因在栽培番茄中的表达情况。系统发育和进化分析表明，被子植物中的SRO基因具有共同的祖先，并且SRO家族成员的数量随着植物的分化和进化而变化。栽培番茄有 6 个 SRO 成员，其中 5 个仍与祖先 SRO 基因具有一定程度的同一性。遗传结构和理化特性表明，番茄SRO基因在染色体分布上高度保守。根据外显子-内含子结构，它们可以分为三类，而栽培番茄仅包含其中两个分支。从番茄SRO基因的启动子中鉴定出许多激素、光和非生物胁迫响应的顺式调控元件，它们还与多种胁迫响应蛋白和microRNA相互作用。 RNA-seq分析表明，SRO基因在番茄不同组织和发育阶段广泛表达，具有显着的组织特异性特征。表达分析还表明，SRO 基因对高温和盐胁迫有显着反应，并介导番茄激素调节网络。这些结果为进一步研究番茄SRO基因的功能表达提供了理论依据，并为番茄抗性育种提供了潜在的遗传资源。
SRO (SIMILAR TO RCD ONE) is a family of plant-specific small molecule proteins that play an important role in plant growth and development and environmental responses. However, SROs still lack systematic characterization in tomato. Based on bioinformatics methods, SRO family genes were identified and characterized from cultivated tomatoes and several wild tomatoes. qRT-PCR was used to study the expression of SRO gene in cultivated tomatoes. Phylogenetic and evolutionary analyses showed that SRO genes in angiosperms share a common ancestor and that the number of SRO family members changed as plants diverged and evolved. Cultivated tomato had six SRO members, five of which still shared some degree of identity with the ancestral SRO genes. Genetic structure and physicochemical properties showed that tomato SRO genes were highly conserved with chromosomal distribution. They could be divided into three groups based on exon-intron structure, and cultivated tomato contained only two of these subclades. A number of hormonal, light and abiotic stress-responsive cis-regulatory elements were identified from the promoter of the tomato SRO gene, and they also interacted with a variety of stress-responsive proteins and microRNAs. RNA-seq analysis showed that SRO genes were widely expressed in different tissues and developmental stages of tomato, with significant tissue-specific features. Expression analysis also showed that SRO genes respond significantly to high temperature and salt stress and mediate the tomato hormone regulatory network. These results provide a theoretical basis for further investigation of the functional expression of tomato SRO genes and provide potential genetic resources for tomato resistance breeding.
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