Investigation into the role of potentially contaminated feed as a source of the first-detected outbreaks of porcine epidemic diarrhea in Canada.
Investigation into the role of potentially contaminated feed as a source of the first-detected outbreaks of porcine epidemic diarrhea in Canada.
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DOI：
10.1111/tbed.12269
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发表时间：
2014-10
影响因子：
4.3
通讯作者：
Alexandersen S
中科院分区：
文献类型：
--
作者：
Pasick J;Berhane Y;Ojkic D;Maxie G;Embury-Hyatt C;Swekla K;Handel K;Fairles J;Alexandersen S
2014 年 1 月，即美国首次发现猪流行性腹泻 (PED) 大约 9 个月后，安大略省西南部的一个猪群中确诊了第一例 PED 病例。对安大略省最初的 PED 病例和随后的 10 例 PED 病例进行的后续流行病学调查表明，饲料是一个常见的危险因素。因此，通过实时 RT-PCR 检测，对多批饲料和用作饲料补充剂的喷雾干燥猪血浆 (SDPP) 进行了 PEDV 基因组的检测。其中一些检测结果呈阳性，支持了受污染的饲料可能是PEDV传入加拿大的原因的观点。这些发现促使我们进行了一项生物测定实验，其中使用三个 PEDV 阳性 SDPP 样品（来自单个批次）和两个补充有该 SDPP 的 PEDV 阳性饲料样品对 3 周龄仔猪进行口服接种。尽管饲料接种的仔猪没有表现出任何明显的 PEDV 排泄，但接种 SDPP 的仔猪在 ≥9 天的时间内以相对较高的水平排出 PEDV。尽管在我们的生物测定实验中，测试的 PEDV 基因组阳性饲料并没有导致明显的仔猪感染，但不能排除受污染的饲料是该领域引入这种病毒的可能来源，因为许多其他变量可能发挥作用。
In January 2014, approximately 9 months following the initial detection of porcine epidemic diarrhea (PED) in the USA, the first case of PED was confirmed in a swine herd in south‐western Ontario. A follow‐up epidemiological investigation carried out on the initial and 10 subsequent Ontario PED cases pointed to feed as a common risk factor. As a result, several lots of feed and spray‐dried porcine plasma (SDPP) used as a feed supplement were tested for the presence of PEDV genome by real‐time RT‐PCR assay. Several of these tested positive, supporting the notion that contaminated feed may have been responsible for the introduction of PEDV into Canada. These findings led us to conduct a bioassay experiment in which three PEDV‐positive SDPP samples (from a single lot) and two PEDV‐positive feed samples supplemented with this SDPP were used to orally inoculate 3‐week‐old piglets. Although the feed‐inoculated piglets did not show any significant excretion of PEDV, the SDPP‐inoculated piglets shed PEDV at a relatively high level for ≥9 days. Despite the fact that the tested PEDV genome positive feed did not result in obvious piglet infection in our bioassay experiment, contaminated feed cannot be ruled out as a likely source of this introduction in the field where many other variables may play a contributing role.
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影响因子：
3.3
作者：
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影响因子：
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影响因子：
3.3
作者：
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Opriessnig, T.
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影响因子：
1.6
作者：
Song D;Park B
通讯作者：
Park B