Isolation of porcine epidemic diarrhea virus in porcine cell cultures and experimental infection of pigs of different ages.
Isolation of porcine epidemic diarrhea virus in porcine cell cultures and experimental infection of pigs of different ages.
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DOI：
10.1016/s0378-1135(99)00199-6
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发表时间：
2000-03-15
影响因子：
3.3
通讯作者：
Uruno K
中科院分区：
文献类型：
--
作者：
Shibata I;Tsuda T;Mori M;Ono M;Sueyoshi M;Uruno K
本文介绍了猪流行性腹泻病毒（PED）在Vero细胞和猪细胞培养中的分离，以及在实验感染中年龄对疾病的影响。从接种PED样品的仔猪小肠中分离PED病毒，并在含有胰蛋白酶的维持培养基（MM）中的胶原包被组织培养板中繁殖的Vero、猪膀胱和肾细胞中培养。在猪膀胱和肾细胞培养物中接种分离的PED病毒，检测到细胞病变效应（CPE），包括细胞融合。在这些细胞的胞质中观察到特异性的明亮荧光。用从暴发中分离的PED病毒经口接种2日龄和7日龄以及2周龄、4周龄、8周龄和12周龄的无特定病原体（SPF）猪。所有接种的2日龄和7日龄猪从接种后第1天（PID）开始出现严重水样腹泻，并在PID 3和4之间死亡。尽管5头2周龄猪中有3头在PID 1-4发生腹泻，但它们最终恢复。在4周龄组中，5头猪中有3头出现轻度腹泻，持续1-2天。8周龄和12周龄猪均未显示任何临床体征。用病毒中和试验（VN）和免疫荧光试验（IFA）检测了所有存活猪的抗PED病毒抗体。因此，猪对致病性PED病毒感染存在年龄依赖性抗性。
This paper describes the isolation of porcine epidemic diarrhea (PED) virus in Vero and porcine cell cultures, and the influence of age on disease in experimental infection. PED virus was isolated from the small intestine of piglets inoculated with PED samples and cultured in Vero, porcine bladder and kidney cells propagated in collagen-coated tissue culture plates in maintenance medium (MM) containing trypsin. In porcine bladder and kidney cell cultures inoculated with isolated PED virus, cytopathic effects (CPE) including cell fusion were detected. Specific brilliant fluorescence was observed in the cytoplasm of these cells. Two- and 7-day old, and 2-, 4-, 8- and 12-week old specific pathogen-free (SPF) pigs were orally inoculated with PED virus isolated from an outbreak. All 2- and 7-day old pigs inoculated developed severe watery diarrhea from post-inoculation day (PID) 1 and died between PID 3 and 4. Although three of five 2-week old pigs developed diarrhea on PID 1–4, they eventually recovered. In the 4-week old group, three of five pigs had mild diarrhea for 1–2 days. None of the 8- and 12-week old pigs showed any clinical signs. Antibodies against PED virus were detected in all surviving pigs by virus neutralization (VN) test and immunofluorescence assay (IFA). Therefore, there is an age-dependent resistance to pathogenic PED virus infection in pigs.
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影响因子：
2.7
作者：
PENSAERT, MB;DEBOUCK, P;REYNOLDS, DJ
通讯作者：
REYNOLDS, DJ
影响因子：
2.4
作者：
COUSSEMENT, W;DUCATELLE, R;HOORENS, J
通讯作者：
HOORENS, J
影响因子：
3.1
作者：
SHIMIZU, M;SHIMIZU, Y;KODAMA, Y
通讯作者：
KODAMA, Y
影响因子：
0.8
作者：
SUEYOSHI, M;TSUDA, T;MORI, M
通讯作者：
MORI, M
影响因子：
2.4
作者：
Chasey D;Cartwright SF
通讯作者：
Cartwright SF