Human limbal niche cells are a powerful regenerative source for the prevention of limbal stem cell deficiency in a rabbit model.
Human limbal niche cells are a powerful regenerative source for the prevention of limbal stem cell deficiency in a rabbit model.
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人类角膜缘微环境细胞是预防兔模型角膜缘干细胞缺陷的强大再生来源
DOI：
10.1038/s41598-018-24862-6
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发表时间：
2018-04-26
影响因子：
4.6
通讯作者：
Zhu Y
中科院分区：
文献类型：
--
作者：
Li G;Zhang Y;Cai S;Sun M;Wang J;Li S;Li X;Tighe S;Chen S;Xie H;Zhu Y
在本文中，使用人角膜缘微环境细胞（LNC）或骨髓来源的间充质干细胞（BMMSC）来预防碱烧伤兔模型中的角膜缘干细胞缺陷（LSCD），并比较了它们的结果。对上皮细胞缺损面积、角膜新生血管和打印细胞计数进行量化，以对 LSCD 的严重程度进行分级。碱烧伤后三个月，对照组（未治疗）出现部分LSCD，表现为慢性角膜上皮缺损、角膜荧光素染色阳性、新生血管形成和杯状细胞迁移。相比之下，LNC 和 BMMSC 移植组的 LSCD 严重程度均显着降低，表现为上皮细胞缺陷更小、荧光素钠染色减少、新血管形成减少和杯状细胞密度降低。有趣的是，LNC 组比 BMMSC 组更能有效预防 LSCD。进一步的分析表明，结膜下移植的 LNC 比 BMMSC 更能预防 LSCD，至少部分是由于 SCF-c-Kit 信号激活的增加。我们得出的结论是，在碱烧伤兔模型中，LNC 是比 BMMSC 更强大的预防 LSCD 的资源，至少部分是由于 SCF 信号激活的增加。
In this article, human limbal niche cells (LNC) or bone marrow derived mesenchymal stem cells (BMMSC) were used to prevent limbal stem cell deficiency (LSCD) in an alkali burn rabbit model and their results were compared. The epithelial cell defect area, corneal neovascularization, and the print cell cytometry were quantified to grade the severity of LSCD. Three months after the alkali burn, a partial LSCD was observed in the control group (no treatment) indicated by chronic corneal epithelial defects, positive corneal fluorescein staining, neovascularization and goblet cell migration. In contrast, the severity of LSCD in both the LNC and BMMSC transplantation groups was dramatically reduced as shown by smaller epithelial cell defects, decreased fluorescein sodium staining, decreased neovascularization and decreased goblet cell density. Interestingly, the LNC group was shown to more effectively prevent LSCD than the BMMSC group. Further analysis indicated subconjunctivally transplanted LNCs were more powerful than BMMSCs to prevent LSCD, at least partially, due to increased activation of SCF-c-Kit signal. We conclude that LNCs are a more powerful resource than BMMSCs to prevent LSCD in an alkali burn rabbit model, at least partially due to increased activation of SCF signaling.
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DOI：
10.1152/ajpendo.00172.2007
发表时间：
2007-08-01
影响因子：
5.1
作者：
Li, Jinming;Quirt, Jaclyn;Wang, Rennian
通讯作者：
Wang, Rennian
影响因子：
10.5
作者：
NOCKA, K;MAJUMDER, S;BESMER, P
通讯作者：
BESMER, P
影响因子：
3.4
作者：
Kanavi, Mozhgan Rezaei;Tabeie, Faraj;AfsarAski, Sasha
通讯作者：
AfsarAski, Sasha
影响因子：
1.8
作者：
OBERG, C;WALTENBERGER, J;WELSH, M
通讯作者：
WELSH, M
影响因子：
0.3
作者：
Kafle, P. A.;Singh, S. K.;Surin, L.
通讯作者：
Surin, L.