Functional Analysis of Stem Cells in the Adult Rat Testis1
Functional Analysis of Stem Cells in the Adult Rat Testis1
复制标题
成年大鼠睾丸干细胞的功能分析1
作者：
K. Orwig;T. Shinohara;M. Avarbock;R. Brinster
摘要成体干细胞在体内维持着几个自我更新的系统和过程，包括表皮、造血、肠上皮和精子发生。然而，对成体干细胞的研究受到其数量少，缺乏有关形态学或生化特征的信息，以及缺乏功能测定的阻碍，除了造血干细胞和精原干细胞。我们利用最近发展的精原细胞移植技术来分析大鼠睾丸的生殖系干细胞。结果表明，大鼠睾丸中的干细胞浓度是小鼠睾丸中干细胞浓度的9.5倍，大鼠供体睾丸细胞移植后3个月的生精细胞集落是小鼠来源集落的2.75倍。因此，在受体睾丸分析时，大鼠干细胞的精子发生程度是小鼠干细胞的26倍。尝试丰富精原干细胞在大鼠睾丸人群中使用实验隐睾程序是不成功的，但选择附着层粘连蛋白包被板导致8.5倍的富集。精原干细胞在成体干细胞中是独特的，因为它们将遗传信息传递给下一代。大鼠睾丸中高浓度的干细胞和移植后精子发生的快速扩增将有利于干细胞生物学研究和将遗传修饰引入雄性生殖系。移植后大鼠与小鼠来源的精原干细胞之间的功能差异表明，这些细胞的潜力可能在物种之间存在很大差异。
Abstract Adult stem cells maintain several self-renewing systems and processes in the body, including the epidermis, hematopoiesis, intestinal epithelium, and spermatogenesis. However, studies on adult stem cells are hampered by their low numbers, lack of information about morphologic or biochemical characteristics, and absence of functional assays, except for hematopoietic and spermatogonial stem cells. We took advantage of the recently developed spermatogonial transplantation technique to analyze germ line stem cells of the rat testis. The results indicate that the stem cell concentration in rat testes is 9.5-fold higher than that in mouse testes, and spermatogenic colonies derived from rat donor testis cells are 2.75 times larger than mouse-derived colonies by 3 mo after transplantation. Therefore, the extent of spermatogenesis from rat stem cells was 26-fold greater than that from mouse stem cells at the time of recipient testis analysis. Attempts to enrich spermatogonial stem cells in rat testis populations using the experimental cryptorchid procedure were not successful, but selection by attachment to laminin-coated plates resulted in 8.5-fold enrichment. Spermatogonial stem cells are unique among adult stem cells because they pass genetic information to the next generation. The high concentration of stem cells in the rat testis and the rapid expansion of spermatogenesis after transplantation will facilitate studies on stem cell biology and the introduction of genetic modifications into the male germ line. The functional differences between spermatogonial stem cells of rat vs. mouse origin after transplantation suggest that the potential of these cells may vary greatly among species.
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影响因子：
--
作者：
L. Russell;R. Brinster
通讯作者：
L. Russell;R. Brinster
影响因子：
56.9
作者：
RHIM, JA;SANDGREN, EP;BRINSTER, RL
通讯作者：
BRINSTER, RL
影响因子：
2.7
作者：
Shinohara, T;Avarbock, MR;Brinster, RL
通讯作者：
Brinster, RL
DOI：
10.1073/pnas.95.8.4356
发表时间：
1998-04-14
影响因子：
11.1
作者：
Kolodka, TM;Garlick, JA;Taichman, LB
通讯作者：
Taichman, LB
DOI：
10.1387/ijdb.9074943
发表时间：
2003-02
期刊：
The International journal of developmental biology
影响因子：
--
作者：
T. Ogawa;J. Aréchaga;M. Avarbock;R. Brinster
通讯作者：
T. Ogawa;J. Aréchaga;M. Avarbock;R. Brinster