Human Umbilical Cord Mesenchymal Stem Cells: A New Therapeutic Option for Tooth Regeneration.
Human Umbilical Cord Mesenchymal Stem Cells: A New Therapeutic Option for Tooth Regeneration.
复制标题
人脐带间充质干细胞：牙齿再生的新治疗选择。
DOI：
10.1155/2015/549432
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发表时间：
2015
影响因子：
4.3
通讯作者：
Liu L
中科院分区：
文献类型：
--
作者：
Chen Y;Yu Y;Chen L;Ye L;Cui J;Sun Q;Li K;Li Z;Liu L
牙齿再生被认为是一种乐观的方法，以取代目前的治疗牙齿脱落。确定最适合牙齿再生的种子细胞是重要的。近年来，人脐带间充质干细胞（human umbilical cord mesenchymal stem cells，hUCMSCs）被认为是一种有希望的组织再生细胞。然而，还没有报道hUCMSCs是否可以用于牙齿再生。在此，我们报道了hUCMSCs可以在体外和体内被诱导为成牙本质细胞样细胞。诱导后的hUCMSCs表达牙本质相关蛋白，包括牙本质涎蛋白（DSP）和牙本质基质蛋白-1（DNT-1），其基因表达水平与天然牙髓组织细胞相似。此外，在体内植入hUCMSC后观察到DSP和β-l阳性钙化。这些结果表明，hUCMSCs具有成牙本质细胞样细胞的分化潜能，在体内具有特征性的牙本质样基质沉积。这项研究清楚地证明了hUCMSC作为牙齿再生的替代治疗性细胞来源。
Tooth regeneration is considered to be an optimistic approach to replace current treatments for tooth loss. It is important to determine the most suitable seed cells for tooth regeneration. Recently, human umbilical cord mesenchymal stem cells (hUCMSCs) have been regarded as a promising candidate for tissue regeneration. However, it has not been reported whether hUCMSCs can be employed in tooth regeneration. Here, we report that hUCMSCs can be induced into odontoblast-like cells in vitro and in vivo. Induced hUCMSCs expressed dentin-related proteins including dentin sialoprotein (DSP) and dentin matrix protein-1 (DMP-1), and their gene expression levels were similar to those in native pulp tissue cells. Moreover, DSP- and DMP-1-positive calcifications were observed after implantation of hUCMSCs in vivo. These findings reveal that hUCMSCs have an odontogenic differentiation potency to differentiate to odontoblast-like cells with characteristic deposition of dentin-like matrix in vivo. This study clearly demonstrates hUCMSCs as an alternative therapeutic cell source for tooth regeneration.
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影响因子：
3.5
作者：
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通讯作者：
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