Electrospray deposition device used to precisely control the matrix crystal to improve the performance of MALDI MSI.
Electrospray deposition device used to precisely control the matrix crystal to improve the performance of MALDI MSI.
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电喷雾沉积装置用于精确控制基质晶体以提高MALDI MSI性能
DOI：
10.1038/srep37903
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发表时间：
2016-11-25
影响因子：
4.6
通讯作者：
Zhao Z
中科院分区：
文献类型：
--
作者：
Li S;Zhang Y;Liu J;Han J;Guan M;Yang H;Lin Y;Xiong S;Zhao Z
MALDI MSI最近已被用作检测特定组织内分子分布的一种创新工具。MALDI MSI需要在感兴趣的组织上沉积一种称为基质的有机化合物，以帮助解吸和电离分析物，其中基质晶体的均匀性和大小对MALDI MSI的成像重复性和空间分辨率有很大影响。在本工作中，我们研制了一种用于MALDI MSI中基质沉积的自制电喷雾沉积装置。该装置可在MALDI微区分析中获得1 μm的均匀基质晶体。首次发现利用电喷雾沉积装置可以精确控制基质晶体的尺寸，并且随着基质晶体尺寸的减小，成像的空间分辨率大大提高。此外，易于制作的电喷雾沉积装置对酸、碱或有机溶剂都具有耐用性，甚至可以用于沉积纳米颗粒基质，这使其在MALDI MSI分析中具有无与伦比的优势。研究了电喷雾沉积装置与MALDI、FTICR、MSI联用分析小鼠脑、肝癌组织切片中脂质分布的可行性。
MALDI MSI has been recently applied as an innovative tool for detection of molecular distribution within a specific tissue. MALDI MSI requires deposition of an organic compound, known as matrix, on the tissue of interest to assist analyte desorption and ionization, in which the matrix crystal homogeneity and size greatly influence the imaging reproducibility and spatial resolution in MALDI MSI. In this work, a homemade electrospray deposition device was developed for deposition of matrix in MALDI MSI. The device could be used to achieve 1 μm homogeneous matrix crystals in MALDI MSI analysis. Moreover, it was found, for the first time, that the electrospray deposition device could be used to precisely control the matrix crystal size, and the imaging spatial resolution was increased greatly as the matrix crystals size becoming smaller. In addition, the easily-built electrospray deposition device was durable for acid, base or organic solvent, and even could be used for deposition of nanoparticles matrix, which made it unparalleled for MALDI MSI analysis. The feasibility of the electrospray deposition device was investigated by combination with MALDI FTICR MSI to analyze the distributions of lipids in mouse brain and liver cancer tissue section.
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影响因子：
14.9
作者：
Wishart DS;Knox C;Guo AC;Eisner R;Young N;Gautam B;Hau DD;Psychogios N;Dong E;Bouatra S;Mandal R;Sinelnikov I;Xia J;Jia L;Cruz JA;Lim E;Sobsey CA;Shrivastava S;Huang P;Liu P;Fang L;Peng J;Fradette R;Cheng D;Tzur D;Clements M;Lewis A;De Souza A;Zuniga A;Dawe M;Xiong Y;Clive D;Greiner R;Nazyrova A;Shaykhutdinov R;Li L;Vogel HJ;Forsythe I
通讯作者：
Forsythe I
聚苯乙烯球辅助基质辅助激光解吸电离质谱法定量分析血浆溶血磷脂酰胆碱
影响因子：
7.4
作者：
Wei, Yanbo;Li, Shumu;Zhao, Zhenwen
通讯作者：
Zhao, Zhenwen
HMDB：人类代谢组数据库。
影响因子：
14.9
作者：
Wishart, David S;Tzur, Dan;Knox, Craig;Eisner, Roman;Guo, An Chi;Young, Nelson;Cheng, Dean;Jewell, Kevin;Arndt, David;Sawhney, Summit;Fung, Chris;Nikolai, Lisa;Lewis, Mike;Coutouly, Marie-Aude;Forsythe, Ian;Tang, Peter;Shrivastava, Savita;Jeroncic, Kevin;Stothard, Paul;Amegbey, Godwin;Block, David;Hau, David D;Wagner, James;Miniaci, Jessica;Clements, Melisa;Gebremedhin, Mulu;Guo, Natalie;Zhang, Ying;Duggan, Gavin E;Macinnis, Glen D;Weljie, Alim M;Dowlatabadi, Reza;Bamforth, Fiona;Clive, Derrick;Greiner, Russ;Li, Liang;Marrie, Tom;Sykes, Brian D;Vogel, Hans J;Querengesser, Lori
通讯作者：
Querengesser, Lori
影响因子：
4.6
作者：
Li T;He J;Mao X;Bi Y;Luo Z;Guo C;Tang F;Xu X;Wang X;Wang M;Chen J;Abliz Z
通讯作者：
Abliz Z
影响因子：
7.4
作者：
Baluya, Dodge L.;Garrett, Timothy J.;Yost, Richard A.
通讯作者：
Yost, Richard A.