Glucose-alanine relationship in normal human pregnancy.
Glucose-alanine relationship in normal human pregnancy.
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正常人类妊娠中的葡萄糖-丙氨酸关系。
DOI：
10.1016/s0026-0495(98)90010-5
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发表时间：
1988
期刊：
影响因子：
--
通讯作者：
Savin,SM
中科院分区：
文献类型：
--
作者：
Kalhan,SC;Gilfillan,CA;Tserng,KY;Savin,SM
为了定量测定正常妊娠人的葡萄糖-丙氨酸关系，对15例妊娠最后4周禁食10小时后的丙氨酸转换率和丙氨酸碳掺入葡萄糖的情况进行了测定。以8例同龄非孕妇女为对照，分别以l-[2，3-13C2]丙氨酸和d[6，6-2H2]葡萄糖为示踪剂。用气相色谱-质谱法测定了丙氨酸、乳酸、葡萄糖的~(13)C富集度和葡萄糖的~(13)H富集度。在5名孕妇和5名非孕妇中，通过间接呼吸量热法和示踪剂输注相结合的方法，评估了丙氨酸碳直接和通过葡萄糖对呼出二氧化碳的贡献。妊娠组与非妊娠组的丙氨酸转换率相似(妊娠组为4.4 3±0.82μμ/kg×m in；非妊娠组为4.11±1.0 8 m ol/kg×m in，均值±SD)。然而，妊娠期间合并到葡萄糖中的丙氨酸比例显著降低(23.5±8.3%vs30.8±8.2%，P<；.04)。在孕妇中，20%的乳酸池来自丙氨酸，而在未怀孕的受试者中，这一比例为28%(P<；0.02)。在怀孕和未怀孕的女性中，24%的丙氨酸转化为二氧化碳。妊娠期血浆胰岛素浓度显著升高(P<；0.05)。这些数据表明，怀孕期间丙氨酸的糖异生作用减弱。这种糖异生的减少不是丙氨酸流量减少的结果，而是由于内在的肝内机制，如主要的胰岛素效应介导的丙氨酸脱氨基减少或肝脏对丙氨酸的摄取减少。人类妊娠中的这些独特的适应性反应导致母体氮的保存，以用于新的母体和胎儿蛋白质的合成。
In order to quantify the glucose-alanine relationship in normal human pregnancy, the turnover rates of alanine and the incorporation of alanine carbon into glucose were quantified in 15 pregnant women during the last 4 weeks of gestation following a ten-hour fast. Eight nonpregnant women of similar age group were studied as controls.l-[2,3-13C2]Alanine andd[6,6-2H2]glucose were infused as tracers. The13C enrichment of alanine, lactate, and glucose and the deuterium enrichment of glucose were measured by gas chromatography-mass spectrometry. In five pregnant and five nonpregnant women, the contribution of alanine carbon to expired CO2directly and via glucose was estimated by combining indirect respiratory calorimetry with the tracer infusions. The alanine turnover rates in the pregnant and nonpregnant women were similar (pregnant, 4.43 ± 0.82 μmol/kg × min; nonpregnant, 4.11 ± 1.08 μmol/kg × min, mean ± SD). However, the fraction of alanine incorporated into glucose was significantly lower during pregnancy (23.5 ± 8.3%v30.8 ± 8.2%,P< .04). In pregnant women, 20% of lactate pool was derived from alanine as compared with 28% in nonpregnant subjects (P< .02). Twenty-four percent of alanine turnover was converted to CO2in both pregnant and nonpregnant women. The plasma insulin concentration was increased significantly during pregnancy (P< .05). These data suggest that gluconeogenesis from alanine is attenuated during pregnancy. This decrease in gluconeogenesis is not the result of decreased alanine flux, but due to intrinsic intrahepatic mechanism such as decreased deamination of alanine mediated by the predominant insulin effect or a decreased hepatic uptake of alanine. These unique adaptive responses in human pregnancy result in conservation of maternal nitrogen for new maternal and fetal protein synthesis.
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DOI：
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影响因子：
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