Differential expression of miRNAs in esophageal cancer tissue.
Differential expression of miRNAs in esophageal cancer tissue.
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DOI：
10.3892/ol.2013.1251
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发表时间：
2013-05
期刊：
影响因子：
2.9
通讯作者：
Wu XN
中科院分区：
文献类型：
--
作者：
Liu SG;Qin XG;Zhao BS;Qi B;Yao WJ;Wang TY;Li HC;Wu XN
本研究旨在通过分析食管癌组织中特异性microRNAs（miRNAs）的表达谱，探讨其与食管癌发生发展的关系，并寻找新的食管癌分子标志物。采用miRNA检测技术检测配对EC及癌旁正常组织中miRNA的表达谱，并进行生物信息学分析。使用实时聚合酶链反应验证结果。miRNA检测结果显示，与癌旁正常组织相比，食管癌组织中共有60条差异表达的miRNA。其中，51个表达量增加了一倍或两倍以上，9个表达量减少了一半。最显著上调的miRNA是hsa-miR-15 a、hsa-miR-28- 3 p、hsa-miR-31、hsa-miR-99 b、hsa-miR-101、hsa-miR-130 a、hsa-miR-143、hsa-miR-196 b、hsa-miR-200 a、hsa-miR-210、hsa-miR-452和hsa-miR-27 a，而最显著下调的miRNA包括hsa-miR-30 b、hsa-miR-223、hsa-miR-454、hsa-miR-223和hsa-miR-27 a。hsa-miR-486、hsa-miR-574-3p和hsa-miR-126。EC组织中存在特异的miRNA表达谱，可能成为新的EC分子标记。
The aim of this study was to explore the association of specific microRNAs (miRNAs) with the development of esophageal cancer (EC) and to identify new molecular markers for EC by analyzing the expression profiles of miRNAs in EC tissues. The expression profiles of miRNAs in paired EC and paracancerous normal tissues were detected and bioinformatically analyzed using miRNA assays. The outcomes were validated using real-time polymerase chain reaction. The miRNA assays revealed a total of 60 differentially expressed miRNAs in the EC tissues compared with those in the paracancerous normal tissues. Among them, 51 had doubled or more than doubled their expression levels and 9 had halved their expression levels. The most markedly upregulated miRNAs were hsa-miR-15a, hsa-miR-28-3p, hsa-miR-31, hsa-miR-99b, hsa-miR-101, hsa-miR-130a, hsa-miR-143, hsa-miR-196b, hsa-miR-200a, hsa-miR-210, hsa-miR-452 and hsa-miR-27a, whereas the most markedly downregulated miRNAs included hsa-miR-30b, hsa-miR-223, hsa-miR-454, hsa-miR-486, hsa-miR-574-3p and hsa-miR-126. Specific miRNA expression profiles exist in EC tissues and may serve as novel EC molecular markers.
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