Luminal bacteria recruit CD103+ dendritic cells into the intestinal epithelium to sample bacterial antigens for presentation.
Luminal bacteria recruit CD103+ dendritic cells into the intestinal epithelium to sample bacterial antigens for presentation.
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DOI：
10.1016/j.immuni.2013.01.009
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发表时间：
2013-03-21
期刊：
影响因子：
32.4
通讯作者：
Shakhar G
中科院分区：
文献类型：
--
作者：
Farache J;Koren I;Milo I;Gurevich I;Kim KW;Zigmond E;Furtado GC;Lira SA;Shakhar G
CD103+树突状细胞(DC)携带来自小肠的细菌，并能将抗原递送给T细胞。然而，它们还没有被记录下来采样肠腔细菌或在肠系膜淋巴结中呈递细菌抗原。我们在活的CX3CR1+/GFP×CD11c-YFP小鼠身上用双光子显微镜研究了这些过程。稳态时，稀疏的CD103+DC占据上皮细胞。他们在肠细胞之间巡逻，同时向管腔延伸树突，可能使用紧密连接蛋白来穿透上皮。沙门氏菌的挑战触发了依赖趋化因子和Toll样受体(TLR)的固有层(LP)中额外的DC的招募。树突状细胞通过上皮内树突有效地吞噬细菌。对非侵入性细菌进行了类似的采样。而CD103+DC对可溶性管腔抗原的采集效率较低。在携带CD103+DC的小鼠中，抗原特异的CD8 T细胞随后在MLN中被激活。因此，肠道CD103+DC具有独特的机制来独立完成细菌抗原的摄取、运输和递呈过程。
CD103+ dendritic cells (DCs) carry bacteria from the small intestine and can present antigens to T cells. Yet they have not been recorded sampling luminal bacteria or presenting bacterial antigens in mesentery lymph nodes. We used 2-photon microscopy in live Cx3cr1+/gfp × Cd11c-YFP mice to study these processes. At steady state, sparse CD103+ DCs occupied the epithelium. They patrolled among enterocytes while extending dendrites toward the lumen, likely using tight-junction proteins to penetrate the epithelium. Challenge with Salmonella triggered chemokine- and toll-like receptor (TLR)-dependent recruitment of additional DCs from the lamina propria (LP). The DCs efficiently phagocytosed the bacteria using intraepithelial dendrites. Noninvasive bacteria were similarly sampled. In contrast, CD103+ DCs sampled soluble luminal antigen inefficiently. In mice harboring CD103+ DCs, antigen-specific CD8 T cells were subsequently activated in MLNs. Intestinal CD103+ DCs are therefore equipped with unique mechanisms to independently complete the processes of uptake, transportation, and presentation of bacterial antigens.
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影响因子：
64.8
作者：
CEPEK, KL;SHAW, SK;BRENNER, MB
通讯作者：
BRENNER, MB
DOI：
10.1083/jcb.100.1.327
发表时间：
1985-01
期刊：
The Journal of cell biology
影响因子：
--
作者：
Boller K;Vestweber D;Kemler R
通讯作者：
Kemler R
影响因子：
4.4
作者：
Huang, Julie H.;Cardenas-Navia, L. Isabel;Dustin, Michael L.
通讯作者：
Dustin, Michael L.
DOI：
10.1083/jcb.129.2.489
发表时间：
1995-04
期刊：
The Journal of cell biology
影响因子：
--
作者：
Hermiston ML;Gordon JI
通讯作者：
Gordon JI
影响因子：
32.4
作者：
Fooksman DR;Schwickert TA;Victora GD;Dustin ML;Nussenzweig MC;Skokos D
通讯作者：
Skokos D