Cross-Stress Adaptation in a Piezophilic and Hyperthermophilic Archaeon From Deep Sea Hydrothermal Vent.
Cross-Stress Adaptation in a Piezophilic and Hyperthermophilic Archaeon From Deep Sea Hydrothermal Vent.
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深海热液喷口嗜压和超嗜热古菌的交叉应力适应
DOI：
10.3389/fmicb.2020.02081
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发表时间：
2020
影响因子：
5.2
通讯作者：
Xiao X
中科院分区：
文献类型：
--
作者：
Zhao W;Ma X;Liu X;Jian H;Zhang Y;Xiao X
嗜热菌生活在80℃以上的环境中，通常伴随着多种极端的环境压力，由于其在生物技术中的应用潜力以及作为原始的现存生命形式而引起了人们的极大关注。对它们的生存和适应机制的研究扩大了我们对生命如何在极端条件下茁壮成长的理解。在这些研究中，人们观察到了各种生物在极端高温和其他环境压力之间的“交叉应激”行为。尽管有广泛的观察，但全球对高温嗜热者交叉应激行为的看法仍然不清楚，这在我们对极端适应的理解上留下了一个知识空白。在本研究中，我们在极端温度、pH、静水压力(HP)和盐度下对一株古生菌热球菌属A501进行了全球定量蛋白质组分析，该菌株在很宽的温度(50-100℃)、pH(4-9)和HPS(0.1-70 Mpa)范围内都有出色的生长能力，但在很小的氯化钠范围(1.0-5.0%，w/v)。蛋白质组学分析(79.8%的基因组覆盖率)表明，约61.5%的显著差异表达蛋白(DEP)对多种胁迫做出了响应。除对高盐度和低温的反应外，对大多数受试胁迫的反应密切相关。前三个丰富的普遍反应过程包括大分子的生物合成和保护、氨基酸的生物合成和代谢、离子转运和结合活性。此外，本研究还揭示了特殊的双重胁迫响应过程，如冷胁迫和高压胁迫下的膜脂以及高渗和热胁迫下的信号转导，以及钠依赖的能量过程，可能是盐度下生长范围的限制因素。本研究首次在蛋白质组学水平上研究了嗜压性嗜热者的整体交叉应激反应。我们的发现为植物对多种胁迫的交叉胁迫适应策略(33.5%的编码基因)提供了直接证据，并突出了对极端温度、pH、盐度和压力的特定和独特的响应过程(每个编码基因的0.22-0.63%)，这与进化生物学和下一代工业生物技术(NGIB)领域高度相关。
Hyperthermophiles, living in environments above 80°C and usually coupling with multi-extreme environmental stresses, have drawn great attention due to their application potential in biotechnology and being the primitive extant forms of life. Studies on their survival and adaptation mechanisms have extended our understanding on how lives thrive under extreme conditions. During these studies, the “cross-stress” behavior in various organisms has been observed between the extreme high temperature and other environmental stresses. Despite the broad observation, the global view of the cross-stress behavior remains unclear in hyperthermophiles, leaving a knowledge gap in our understanding of extreme adaptation. In this study, we performed a global quantitative proteomic analysis under extreme temperatures, pH, hydrostatic pressure (HP), and salinity on an archaeal strain, Thermococcus eurythermalis A501, which has outstanding growth capability on a wide range of temperatures (50–100°C), pH (4–9), and HPs (0.1–70 MPa), but a narrow range of NaCl (1.0–5.0 %, w/v). The proteomic analysis (79.8% genome coverage) demonstrated that approximately 61.5% of the significant differentially expressed proteins (DEPs) responded to multiple stresses. The responses to most of the tested stresses were closely correlated, except the responses to high salinity and low temperature. The top three enriched universal responding processes include the biosynthesis and protection of macromolecules, biosynthesis and metabolism of amino acids, ion transport, and binding activities. In addition, this study also revealed that the specific dual-stress responding processes, such as the membrane lipids for both cold and HP stresses and the signal transduction for both hyperosmotic and heat stresses, as well as the sodium-dependent energetic processes might be the limiting factor of the growth range in salinity. The present study is the first to examine the global cross-stress responses in a piezophilic hyperthermophile at the proteomic level. Our findings provide direct evidences of the cross-stress adaptation strategy (33.5% of coding-genes) to multiple stresses and highlight the specific and unique responding processes (0.22–0.63% of coding genes for each) to extreme temperature, pH, salinity, and pressure, which are highly relevant to the fields of evolutionary biology as well as next generation industrial biotechnology (NGIB).
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影响因子：
5.7
作者：
Hou, Longyu;Wang, Mingya;Mao, Peisheng
通讯作者：
Mao, Peisheng
影响因子：
3.3
作者：
Boyd, Eric S.
通讯作者：
Boyd, Eric S.
DOI：
10.1186/1746-1448-3-6
发表时间：
2007-07-25
期刊：
Saline systems
影响因子：
--
作者：
Coker JA;DasSarma P;Kumar J;Müller JA;DasSarma S
通讯作者：
DasSarma S
影响因子：
--
作者：
Jain, Samta;Caforio, Antonella;Driessen, Arnold J. M.
通讯作者：
Driessen, Arnold J. M.
影响因子：
5.2
作者：
Cario A;Grossi V;Schaeffer P;Oger PM
通讯作者：
Oger PM