Standing surface acoustic wave (SSAW)-based microfluidic cytometer.
Standing surface acoustic wave (SSAW)-based microfluidic cytometer.
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DOI：
10.1039/c3lc51139a
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发表时间：
2014-03-07
期刊：
影响因子：
6.1
通讯作者：
Huang TJ
中科院分区：
文献类型：
--
作者：
Chen Y;Nawaz AA;Zhao Y;Huang PH;McCoy JP;Levine SJ;Wang L;Huang TJ
基于微流控芯片的流式细胞仪因其体积小、成本低等优点而成为一个重要的研究领域。在此，我们展示了一种无鞘微流控流式细胞仪，该流式细胞仪集成了能够进行3D颗粒/细胞聚焦的基于表面声波（SSAW）的微型装置与激光诱导荧光（LIF）检测系统。使用SSAW，我们的微流控细胞仪能够在微通道内的压力节点处连续聚焦微粒/细胞。使用该系统成功证明了流式细胞术，当使用校准珠时，在约1000个事件/s的通量下变异系数（CV）小于10%。我们还证明，荧光标记的人早幼粒细胞白血病细胞（HL-60）可以有效地集中和检测与我们的SSAW为基础的系统。这种基于SSAW的微流体细胞仪不需要任何鞘流或复杂的结构，并且它允许在宽范围的样品流速下进行简单的操作。此外，由于低功率表面声波具有温和的生物相容性，这种技术有望保持细胞和其他生物颗粒的完整性。
The development of microfluidic chip-based cytometers has become an important area due to their advantages of compact size and low cost. Herein, we demonstrate a sheathless microfluidic cytometer which integrates a standing surface acoustic wave (SSAW)-based microdevice capable of 3D particle/cell focusing with a laser-induced fluorescence (LIF) detection system. Using SSAW, our microfluidic cytometer was able to continuously focus microparticles/cells at the pressure node inside a microchannel. Flow cytometry was successfully demonstrated using this system with a coefficient of variation (CV) of less than 10% at a throughput of ~1000 events/s when calibration beads were used. We also demonstrated that fluorescently labeled human promyelocytic leukemia cells (HL-60) could be effectively focused and detected with our SSAW-based system. This SSAW-based microfluidic cytometer did not require any sheath flows or complex structures, and it allowed for simple operation over a wide range of sample flow rates. Moreover, with the gentle, bio-compatible nature of low-power surface acoustic waves, this technique is expected to be able to preserve the integrity of cells and other bioparticles.
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影响因子：
12.6
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