Nerve Guidance Conduits with Hierarchical Anisotropic Architecture for Peripheral Nerve Regeneration.
Nerve Guidance Conduits with Hierarchical Anisotropic Architecture for Peripheral Nerve Regeneration.
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DOI：
10.1002/adhm.202100427
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发表时间：
2021-07
影响因子：
10
通讯作者：
Kaplan DL
中科院分区：
文献类型：
--
作者：
Lu Q;Zhang F;Cheng W;Gao X;Ding Z;Zhang X;Lu Q;Kaplan DL
具有多功能特征的神经引导导管可以为改善神经再生和功能恢复提供微环境。然而，由于制造过程中这些因素的相互作用，优化神经导管系统中的多个线索仍然是一个挑战。在这里，用于制造神经导管的模块化组件被用于解决结合用于神经再生的多功能引导线索的目标。开发了具有合适尺寸和机械性能的丝基中空导管，沿着具有可调分级各向异性结构和微孔结构的丝填料，并组装成导管。这些导管支持在体外细胞增殖（Schwann和PC12细胞）和生长因子分泌（BDNF，脑源性神经营养因子）方面改善神经再生，以及大鼠坐骨神经缺损的体内修复和功能恢复。使用这些新导管设计的神经再生与自体移植物相当，为未来的临床影响提供了一条途径。通过模块化组装工艺制造了含有具有可调分层各向异性结构的丝素填充物的丝基神经导管。与自体神经移植物相比，该导管在体外促进雪旺细胞增殖和PC12细胞轴突生长，在体内也加速大鼠坐骨神经缺损的修复和功能恢复。
Nerve guidance conduits with multifunctional features could offer microenvironments for improved nerve regeneration and functional recovery. However, the challenge remains to optimize multiple cues in nerve conduit systems due to the interplay of these factors during fabrication. Here, a modular assembly for the fabrication of nerve conduits was utilized to address the goal of incorporating multifunctional guidance cues for nerve regeneration. Silk-based hollow conduits with suitable size and mechanical properties, along with silk nanofiber fillers with tunable hierarchical anisotropic architectures and microporous structures, were developed and assembled into conduits. These conduits supported improved nerve regeneration in terms of cell proliferation (Schwann and PC12 cells) and growth factor secretion (BDNF, brain-derived neurotrophic factor) in vitro, and the in vivo repair and functional recovery of rat sciatic nerve defects. Nerve regeneration using these new conduit designs was comparable to autografts, providing a path towards future clinical impact. Silk-based nerve conduits containing silk nanofiber fillers with tunable hierarchical anisotropic architectures were fabricated through a modular assembly process. The conduits promoted the proliferation of schwann cells and the axonal outgrowth of PC12 cells in vitro and also accelerated the repair and functional recovery of rat sciatic nerve defects in vivo, comparable to autografts.
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影响因子：
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作者：
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作者：
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通讯作者：
Amdursky, Nadav
影响因子：
19
作者：
Grasman, Jonathan M.;Ferreira, Julia A.;Kaplan, David L.
通讯作者：
Kaplan, David L.