Development of Biopolymeric Hybrid Scaffold-Based on AAc/GO/nHAp/TiO(2) Nanocomposite for Bone Tissue Engineering: In-Vitro Analysis.
Development of Biopolymeric Hybrid Scaffold-Based on AAc/GO/nHAp/TiO(2) Nanocomposite for Bone Tissue Engineering: In-Vitro Analysis.
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DOI：
10.3390/nano11051319
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发表时间：
2021-05-17
期刊：
影响因子：
--
通讯作者：
Amin R
中科院分区：
文献类型：
--
作者：
Aslam Khan MU;Al-Arjan WS;Binkadem MS;Mehboob H;Haider A;Raza MA;Abd Razak SI;Hasan A;Amin R
骨组织工程是治疗骨折以恢复/调节生物功能的前沿领域。生物聚合物/生物陶瓷杂化纳米复合支架具有生物可降解性和生物相容性，是一种潜在的骨组织生物材料。报道了以丙烯酸(AAc)/瓜尔豆胶(GG)、纳米羟基磷灰石(HAP NPs)、纳米二氧化钛(TiO2NPs)为主要原料，采用自由基聚合法制备纳米复合材料，并优化了氧化石墨烯(GO)的用量。采用冷冻干燥法制备了多孔支架，并在支架表面涂覆了磺胺嘧啶银。使用不同的技术研究了所制备的支架的官能团、晶体结构特性、形态/元素特性、孔隙率和力学性能。结果表明，随着二氧化钛加入量的增加，材料的物化性能和微观结构性能、力学性能(抗压强度(2.96Mpa~13.31Mpa)和杨氏弹性模量(39.56Mpa~300.81 Mpa))和孔性能(孔径(256.11~107.42μm)和孔隙率(79.97%~44.32%)都得到了改善。150min后，磺胺嘧啶银的释放率为94.1%。支架的体外实验也显示了对小鼠前成骨细胞(MC3T3-E1)细胞株的良好结果。因此，这些支架材料有望成为生物医学工程中骨组织工程的潜在生物材料。
Bone tissue engineering is an advanced field for treatment of fractured bones to restore/regulate biological functions. Biopolymeric/bioceramic-based hybrid nanocomposite scaffolds are potential biomaterials for bone tissue because of biodegradable and biocompatible characteristics. We report synthesis of nanocomposite based on acrylic acid (AAc)/guar gum (GG), nano-hydroxyapatite (HAp NPs), titanium nanoparticles (TiO2 NPs), and optimum graphene oxide (GO) amount via free radical polymerization method. Porous scaffolds were fabricated through freeze-drying technique and coated with silver sulphadiazine. Different techniques were used to investigate functional group, crystal structural properties, morphology/elemental properties, porosity, and mechanical properties of fabricated scaffolds. Results show that increasing amount of TiO2 in combination with optimized GO has improved physicochemical and microstructural properties, mechanical properties (compressive strength (2.96 to 13.31 MPa) and Young’s modulus (39.56 to 300.81 MPa)), and porous properties (pore size (256.11 to 107.42 μm) and porosity (79.97 to 44.32%)). After 150 min, silver sulfadiazine release was found to be ~94.1%. In vitro assay of scaffolds also exhibited promising results against mouse pre-osteoblast (MC3T3-E1) cell lines. Hence, these fabricated scaffolds would be potential biomaterials for bone tissue engineering in biomedical engineering.
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DOI：
10.3390/molecules26030619
发表时间：
2021-01-25
期刊：
Molecules (Basel, Switzerland)
影响因子：
--
作者：
Aslam Khan MU;Abd Razak SI;Al Arjan WS;Nazir S;Sahaya Anand TJ;Mehboob H;Amin R
通讯作者：
Amin R
影响因子：
4.1
作者：
Khan MUA;Abd Razak SI;Mehboob H;Abdul Kadir MR;Anand TJS;Inam F;Shah SA;Abdel-Haliem MEF;Amin R
通讯作者：
Amin R
DOI：
10.1016/j.ijbiomac.2017.03.067
发表时间：
2017-08-01
影响因子：
8.2
作者：
Abd-Khorsand, Saber;Saber-Samandari, Samaneh;Saber-Samandari, Saeed
通讯作者：
Saber-Samandari, Saeed
影响因子：
0.9
作者：
Bouropoulos, Nikolaos;Stampolakis, Alexandros;Mouzakis, Dionysios E.
通讯作者：
Mouzakis, Dionysios E.
影响因子：
5.4
作者：
Civantos, Ana;Dominguez, Cristina;Torres, Yadir
通讯作者：
Torres, Yadir