Expression of secreted Wnt pathway components reveals unexpected complexity of the planarian amputation response.
Expression of secreted Wnt pathway components reveals unexpected complexity of the planarian amputation response.
复制标题
DOI：
10.1016/j.ydbio.2010.08.007
复制
发表时间：
2010-11-01
影响因子：
2.7
通讯作者：
Sánchez Alvarado A
中科院分区：
文献类型：
--
作者：
Gurley KA;Elliott SA;Simakov O;Schmidt HA;Holstein TW;Sánchez Alvarado A
再生在整个动物界都很普遍，但我们对成年动物中这一过程的分子理解仍然很不清楚。Wnt/β-连环蛋白信号传导在动物从早期发育到成年的整个生命过程中发挥着至关重要的作用。在完整和再生的真涡虫中，Wnt/β-catenin信号传导的调节功能是维持和指定前/后（A/P）身份。在这里，我们探讨的表达动力学和RNAi表型的分泌成员的Wnt信号通路中的涡虫Schmidtea mediterranea。Smed-Wnt和sFRP在再生过程中的表达是令人惊讶的动态，并揭示了先前未被重视的Planarian生物学的基本方面。我们发现，截肢后，受伤的反应之前的A/P轴的快速重组。此外，整个身体计划的细胞可以安装这种反应，并重新评估其新的A/P位置在完全没有干细胞的情况下。虽然截肢反应的初始阶段是干细胞无关的，但组织重塑和新A/P地址与解剖结构的整合是干细胞依赖的。我们还表明，WNT 5功能在一个互惠的方式与SLIT图案的涡虫mediolateral轴，而WNT 11 -2图案后中线。此外，我们进行了广泛的系统发育分析的Smed-wnt基因使用的方法，结合和整合序列和结构比对，使我们能够将所有9个基因到Wnt亚家族的第一次。
Regeneration is widespread throughout the animal kingdom, but our molecular understanding of this process in adult animals remains poorly understood. Wnt/β-catenin signaling plays crucial roles throughout animal life from early development to adulthood. In intact and regenerating planarians, the regulation of Wnt/β-catenin signaling functions to maintain and specify anterior/posterior (A/P) identity. Here, we explore the expression kinetics and RNAi phenotypes for secreted members of the Wnt signaling pathway in the planarian Schmidtea mediterranea. Smed-wnt and sFRP expression during regeneration is surprisingly dynamic and reveals fundamental aspects of planarian biology that have been previously unappreciated. We show that after amputation, a wounding response precedes rapid reorganization of the A/P axis. Furthermore, cells throughout the body plan can mount this response and reassess their new A/P location in the complete absence of stem cells. While initial stages of the amputation response are stem cell independent, tissue remodeling and the integration of new A/P address with anatomy are stem cell dependent. We also show that WNT5 functions in a reciprocal manner with SLIT to pattern the planarian mediolateral axis, while WNT11-2 patterns the posterior midline. Moreover, we perform an extensive phylogenetic analysis on the Smed-wnt genes using a method that combines and integrates both sequence and structural alignments, enabling us to place all nine genes into Wnt subfamilies for the first time.
登录
查看更多内容
影响因子：
2.7
作者：
Augustin, Rene;Franke, Andre;Bosch, Thomas C. G.
通讯作者：
Bosch, Thomas C. G.
影响因子：
2.7
作者：
Fradkin, LG;van Schie, M;Noordermeer, JN
通讯作者：
Noordermeer, JN
影响因子：
64.8
作者：
Hobmayer, B;Rentzsch, F;Holstein, TW
通讯作者：
Holstein, TW
影响因子：
56.9
作者：
Gurley, Kyle A.;Rink, Jochen C.;Alvarado, Alejandro Sanchez
通讯作者：
Alvarado, Alejandro Sanchez
影响因子：
4.6
作者：
Iglesias, Marta;Luis Gomez-Skarmeta, Jose;Adell, Teresa
通讯作者：
Adell, Teresa