FAST (Flexible Acetylcholine Sensing Thread): Real-Time Detection of Acetylcholine with a Flexible Solid-Contact Potentiometric Sensor.
FAST (Flexible Acetylcholine Sensing Thread): Real-Time Detection of Acetylcholine with a Flexible Solid-Contact Potentiometric Sensor.
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DOI：
10.3390/bioengineering10060655
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发表时间：
2023-05-27
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乙酰胆碱（ACh）参与记忆和学习，并与神经退行性疾病有关；因此，研究乙酰胆碱在大脑中的动态是很重要的。这项工作创造了一种灵活的固体接触电位传感器，用于体外和体内记录大脑和组织匀浆中的乙酰胆碱。我们使用直径250 μm的棉纱和含有杯状[4]芳烃离子载体的ACh传感膜来制造该传感器，棉纱表面涂有柔性导电油墨。暴露的离子电子传感器用2.5 μm厚的聚对二甲苯C涂层密封，以保持传感器的灵活性。所得的柔性ACh传感螺纹（FAST）直径为400 μm。FAST在去离子水中的线性响应范围为1.0 μM ~ 10.0 mM，近似纳氏斜率为56.11 mV/ 10年，检测限为2.6 μM。在人工脑脊液中，由于脑脊液离子含量的背景信号，检测限提高到20 μM。FAST在24 h内的信号稳定性为226 μV/h。结果表明，FAST可以测量ACh峰值后绵羊脑组织和脑组织匀浆中的ACh动态。FAST是第一个用于监测大脑中乙酰胆碱动力学的柔性电化学传感器。
Acetylcholine (ACh) is involved in memory and learning and has implications in neurodegenerative diseases; it is therefore important to study the dynamics of ACh in the brain. This work creates a flexible solid-contact potentiometric sensor for in vitro and in vivo recording of ACh in the brain and tissue homogenate. We fabricate this sensor using a 250 μm diameter cotton yarn coated with a flexible conductive ink and an ACh sensing membrane that contains a calix[4]arene ionophore. The exposed ion-to-electron transducer was sealed with a 2.5 μm thick Parylene C coating to maintain the flexibility of the sensor. The resulting diameter of the flexible ACh sensing thread (FAST) was 400 μm. The FAST showed a linear response range from 1.0 μM to 10.0 mM in deionized water, with a near-Nernstian slope of 56.11 mV/decade and a limit of detection of 2.6 μM. In artificial cerebrospinal fluid, the limit of detection increased to 20 μM due to the background signal of ionic content of the cerebrospinal fluid. The FAST showed a signal stability of 226 μV/h over 24 h. We show that FAST can measure ACh dynamics in sheep brain tissue and sheep brain homogenate after ACh spiking. FAST is the first flexible electrochemical sensor for monitoring ACh dynamics in the brain.
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