Species specific anaesthetics for fish anaesthesia and euthanasia.
Species specific anaesthetics for fish anaesthesia and euthanasia.
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DOI：
10.1038/s41598-017-06917-2
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发表时间：
2017-08-02
影响因子：
4.6
通讯作者：
Murrell JC
中科院分区：
文献类型：
--
作者：
Readman GD;Owen SF;Knowles TG;Murrell JC
有必要确保实验室对鱼类的护理和福利达到最高标准。这延伸到麻醉药在科学研究、人道杀戮和生命结束时的安乐死的使用。麻醉药不应引起不良行为，鱼不应试图避免麻醉药。令人惊讶的是，尽管麻醉药的使用已有100多年，全世界数千个实验室中目前保存着数百万条鱼，但可供人类选择麻醉药的信息却很少。使用化学趋化选择室，我们发现在实验室中常见的四种密切相关的鱼类中，暴露于三种广泛使用的麻醉剂中，不同的物种特定行为反应。正如之前在斑马鱼（Danio rerio）中发现的那样，MS-222和苯佐卡因的使用似乎也会诱导米达卡鱼（Oryzias latipes）的回避行为；但依托咪酯可以提供另一种选择。鲤鱼（Cyprinus carpio）与斑马鱼有亲缘关系，但对乙咪酯有回避行为，对苯佐卡因和MS-222无回避行为；虹鳟（Oncorhynchus mykiss）对三种药剂均无回避反应。我们无法确定黑头鲦鱼（Pimephales promelas）的回避反应，并建议对该物种需要不同的测试范式。
There is a need to ensure that the care and welfare for fish maintained in the laboratory are to the highest standards. This extends to the use of anaesthetics for both scientific study, humane killing and euthanasia at end of life. An anaesthetic should not induce negative behaviours and fish should not seek to avoid the anaesthetic. Surprisingly little information is available to facilitate a humane choice of anaesthetic agent for fish despite over 100 years of use and the millions of fish currently held in thousands of laboratories worldwide. Using a chemotaxic choice chamber we found different species specific behavioural responses among four closely related fish species commonly held in the laboratory, exposed to three widely used anaesthetic agents. As previously found for zebrafish (Danio rerio), the use of MS-222 and benzocaine also appears to induce avoidance behaviours in medaka (Oryzias latipes); but etomidate could provide an alternative choice. Carp (Cyprinus carpio), although closely related to zebrafish showed avoidance behaviours to etomidate, but not benzocaine or MS-222; and rainbow trout (Oncorhynchus mykiss) showed no avoidance to the three agents tested. We were unable to ascertain avoidance responses in fathead minnows (Pimephales promelas) and suggest different test paradigms are required for that species.
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影响因子：
3.7
作者：
Wong, Devina;von Keyserlingk, Marina A. G.;Weary, Daniel M.
通讯作者：
Weary, Daniel M.
影响因子：
1.7
作者：
Stockman, Jonathan;Weber, Ernest Scott P., III;Paul-Murphy, Joanne
通讯作者：
Paul-Murphy, Joanne
影响因子：
2.9
作者：
Zahl, Inger Hilde;Kiessling, Anders;Olsen, Rolf Erik
通讯作者：
Olsen, Rolf Erik
影响因子：
2.4
作者：
KORVER, RM;SPRAGUE, JB
通讯作者：
SPRAGUE, JB
影响因子：
2.9
作者：
Zahl, Inger Hilde;Samuelsen, Ole;Kiessling, Anders
通讯作者：
Kiessling, Anders