Prevalence of Crimean-Congo haemorrhagic fever in livestock following a confirmed human case in Lyantonde district, Uganda.
Prevalence of Crimean-Congo haemorrhagic fever in livestock following a confirmed human case in Lyantonde district, Uganda.
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DOI：
10.1186/s13071-022-05588-x
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发表时间：
2023-01-07
影响因子：
3.2
通讯作者：
Thomson, Emma C.
中科院分区：
文献类型：
--
作者：
Atim, Stella A.;Niebel, Marc;Ashraf, Shirin;Vudriko, Patrick;Odongo, Steven;Balinandi, Stephen;Aber, Peace;Bameka, Ronald;Ademun, Anna R.;Masembe, Charles;Tweyongyere, Robert;Thomson, Emma C.
关键词：
克里米亚-刚果出血热（CCHF）是一种蜱传病毒感染，其特征是人类出血热和动物短暂无症状感染。这是一种新出现的人类健康威胁，导致乌干达零星爆发。乌干达利安通德区一名 42 岁的男性牛贸易商死于 CCHF 后，我们对动物种群进行了详细的疫情调查。这是为了确定 CCHF 病毒 (CCHFV) 在牛和山羊中的传播程度，并确定受影响的农场以及未来人类感染的持续增加的环境风险。我们收集了 117 头牛和 93 只山羊的血液和蜱样本，并使用酶联免疫吸附测定 (ELISA)、定量逆转录酶聚合酶链反应 (qRT-PCR) 和目标富集下一代测序来测试这些样本的抗 CCHFV 抗体和抗原。在 110/117 (94.0%) 牛和 83/93 (89.3%) 山羊中检测到 CCHFV 特异性 IgG 抗体。动物血清阳性与雌性动物独立相关（AOR = 9.42，P = 0.002），在畜牧生产系统下饲养的动物（AOR = 6.02，P = 0.019）比拴系或公共放牧的动物更可能出现血清阳性。CCHFV是通过测序检测到的附肢扇头蜱存在，但在家畜中没有。这项调查表明，感染率非常高。 与 Lyantonde 人类 CCHF 病例相关的农场中牛和山羊的 CCHFV 抗体血清阳性率。因此，建议在该地区农场和乌干达养牛走廊周围建立 CCHF 监测计划，以确定病例预防和控制的机会。在线版本包含可在 10.1186/s13071-022-05588-x 获取的补充材料。
Crimean-Congo haemorrhagic fever (CCHF) is a tick-borne viral infection, characterized by haemorrhagic fever in humans and transient asymptomatic infection in animals. It is an emerging human health threat causing sporadic outbreaks in Uganda. We conducted a detailed outbreak investigation in the animal population following the death from CCHF of a 42-year-old male cattle trader in Lyantonde district, Uganda. This was to ascertain the extent of CCHF virus (CCHFV) circulation among cattle and goats and to identify affected farms and ongoing increased environmental risk for future human infections. We collected blood and tick samples from 117 cattle and 93 goats, and tested these for anti-CCHFV antibodies and antigen using an enzyme-linked immunosorbent assay (ELISA), quantitative reverse transcriptase polymerase chain reaction (qRT-PCR) and target enrichment next generation sequencing. CCHFV-specific IgG antibodies were detected in 110/117 (94.0%) cattle and 83/93 (89.3%) goats. Animal seropositivity was independently associated with female animals (AOR = 9.42, P = 0.002), and animals reared under a pastoral animal production system (AOR = 6.02, P = 0.019] were more likely to be seropositive than tethered or communally grazed animals. CCHFV was detected by sequencing in Rhipicephalus appendiculatus ticks but not in domestic animals. This investigation demonstrated very high seroprevalence of CCHFV antibodies in both cattle and goats in farms associated with a human case of CCHF in Lyantonde. Therefore, building surveillance programs for CCHF around farms in this area and the Ugandan cattle corridor is indicated, in order to identify opportunities for case prevention and control. The online version contains supplementary material available at 10.1186/s13071-022-05588-x.
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影响因子：
33.9
作者：
Kizito, Susan;Okello, Paul E.;Zhu, Bao-Ping
通讯作者：
Zhu, Bao-Ping
影响因子：
3.7
作者：
Lista MJ;Matos PM;Maguire TJA;Poulton K;Ortiz-Zapater E;Page R;Sertkaya H;Ortega-Prieto AM;Scourfield E;O'Byrne AM;Bouton C;Dickenson RE;Ficarelli M;Jimenez-Guardeño JM;Howard M;Betancor G;Galao RP;Pickering S;Signell AW;Wilson H;Cliff P;Kia Ik MT;Patel A;MacMahon E;Cunningham E;Doores K;Agromayor M;Martin-Serrano J;Perucha E;Mischo HE;Shankar-Hari M;Batra R;Edgeworth J;Zuckerman M;Malim MH;Neil S;Martinez-Nunez RT
通讯作者：
Martinez-Nunez RT
影响因子：
158.5
作者：
Negredo, A.;de la Calle-Prieto, F.;Arribas, J. R.
通讯作者：
Arribas, J. R.
影响因子：
3.1
作者：
Balinandi, Stephen;von Bromssen, Claudia;Malmberg, Maja
通讯作者：
Malmberg, Maja
影响因子：
2.1
作者：
Atkinson, Barry;Chamberlain, John;Hewson, Roger
通讯作者：
Hewson, Roger