Chlorine Dioxide Inhibits African Swine Fever Virus by Blocking Viral Attachment and Destroying Viral Nucleic Acids and Proteins.
Chlorine Dioxide Inhibits African Swine Fever Virus by Blocking Viral Attachment and Destroying Viral Nucleic Acids and Proteins.
复制标题
二氧化氯通过阻断病毒附着并破坏病毒核酸和蛋白质来抑制非洲猪瘟病毒
DOI：
10.3389/fvets.2022.844058
复制
发表时间：
2022
影响因子：
3.2
通讯作者：
Guo C
中科院分区：
文献类型：
--
作者：
Wei R;Wang X;Cao Y;Gong L;Liu X;Zhang G;Guo C
非洲猪瘟（ASF）是一种高度传染性疾病，会造成严重的经济损失和健康威胁。目前尚无有效的疫苗或治疗方法来预防或治愈非洲猪瘟。因此，迫切需要开发针对非洲猪瘟病毒（ASFV）的有效药物。二氧化氯（ClO2）是一种理想的杀菌剂，具有广谱抗菌活性，且无耐药性。在这里，我们发现 ClO2 强烈抑制猪肺泡巨噬细胞 (PAM) 中的 ASFV 复制。 ClO2 的抑制作用发生在病毒附着而非进入期间，表明 ClO2 抑制了病毒生命周期的早期阶段。当在病毒感染之前、同时或之后添加时，ClO2 显示出有效的抗 ASFV 效果。此外，ClO2 可以破坏病毒核酸和蛋白质，这可能有助于其灭活 ASFV 病毒体的能力。 ClO2降解ASFV核酸的最低浓度为1.2 μg/mL，且降解方式呈温度依赖性。这些对猪场二氧化氯防治非洲猪瘟病毒感染具有指导意义。此外，ClO2 还降低了 ASFV 诱导的炎症细胞因子的表达。总的来说，我们的研究结果表明，ClO2 可能是养猪业开发新型抗 ASFV 预防和治疗药物的理想候选药物。
African swine fever (ASF) is a highly contagious disease and provokes severe economic losses and health threats. At present no effective vaccine or treatment is available to prevent or cure ASF. Consequently, there is an urgent need to develop effective drugs against ASF virus (ASFV). Chlorine dioxide (ClO2), an ideal biocide, has broad-spectrum antibacterial activity and no drug resistance. Here, we found that ClO2 strongly inhibited ASFV replication in porcine alveolar macrophages (PAMs). The inhibitory effect of ClO2 occurred during viral attachment rather than entry, indicating that ClO2 suppressed the early stage of virus life cycle. ClO2 showed a potent anti-ASFV effect when added either before, simultaneously with, or after virus infection. Furthermore, ClO2 could destroy viral nucleic acids and proteins, which may contribute to its capacity of inactivating ASFV virions. The minimum concentration of degradation of ASFV nucleic acids by ClO2 is 1.2 μg/mL, and the degradation is a temperature-dependent manner. These have guiding significance for ClO2 prevention and control of ASFV infection in pig farms. In addition, ClO2 decreased the expression of ASFV-induced inflammatory cytokines. Overall, our findings suggest that ClO2 may be an ideal candidate for the development of novel anti-ASFV prophylactic and therapeutic drugs in swine industry.
登录
查看更多内容
DOI：
10.1073/pnas.1922523117
发表时间：
2020-05-19
影响因子：
11.1
作者：
Liu, Ruili;Sun, Yeping;Wang, Han
通讯作者：
Wang, Han
DOI：
10.1016/j.msec.2019.01.043
发表时间：
2019-05-01
影响因子：
7.9
作者：
Palcso, Barnabas;Moldovan, Zsofia;Zelko, Romana
通讯作者：
Zelko, Romana
影响因子：
3.2
作者：
Zhu, Zhenbang;Guo, Yang;Guo, Chunhe
通讯作者：
Guo, Chunhe
影响因子：
6.7
作者：
Sánchez EG;Quintas A;Pérez-Núñez D;Nogal M;Barroso S;Carrascosa ÁL;Revilla Y
通讯作者：
Revilla Y
影响因子：
4.3
作者：
Sanna, G.;Dei Giudici, S.;Oggiano, A.
通讯作者：
Oggiano, A.