Sustained Release of Antimicrobial Peptide from Self-Assembling Hydrogel Enhanced Osteogenesis
Sustained Release of Antimicrobial Peptide from Self-Assembling Hydrogel Enhanced Osteogenesis
复制标题
自组装水凝胶持续释放抗菌肽增强成骨作用
DOI：
10.1080/09205063.2018.1504191
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发表时间：
2018-09
期刊：
影响因子：
--
通讯作者：
Lingling Dong
中科院分区：
文献类型：
--
作者：
Guoli Yang;Tingben Huang;Ying Wang;Huiming Wang;Yongzheng Li;Ke Yu;Lingling Dong
摘要生物材料因其多功能性而被广泛应用于骨感染和骨髓炎的治疗。据我们所知，骨髓炎的出现主要是由细菌引起的。因此，一种既能治愈骨感染又能促进成骨的生物材料有可能成为治疗骨髓炎的理想材料。阳离子抗菌肽（Amp）已被证明具有良好的杀灭细菌、真菌、病毒和寄生虫的能力。但抗菌肽在骨感染和骨髓炎中的应用还很有限。在这里，我们设计了一种新的水凝胶，对S。金黄色葡萄球菌并增强骨生成。RADA 16自组装肽已被应用于AMP递送。在本研究中，我们证明了RADA 16可以形成稳定的结构，并提供AMP的持续释放。通过场发射扫描电子显微镜检测了交织的微结构。缓释研究表明，AMP的释放可持续至28天。体外研究表明，这种新型自组装水凝胶能够促进骨髓间充质干细胞（BMSCs）的增殖，抑制S。金黄色。更重要的是，体内实验结果也证明了RADA 16-AMP自组装肽对骨形成具有优异的作用。我们的研究结果表明，我们成功地将RADA 16和AMP结合在一起，为这种新型水凝胶的应用奠定了基础，并为生物材料开辟了新的途径。
Abstract Biomaterials have been widely used in bone infection and osteomyelitis resulting from their versatile functionalities. As far as we know, the appearance of osteomyelitis was mainly caused by bacteria. Therefore, a biomaterial that can cure bone infection and promote osteogenesis may become an ideal candidate for the treatment of osteomyelitis. Cationic antimicrobial peptides (AMPs) have been proved to have an excellent ability to kill bacteria, fungi, viruses, and parasites. However, the application of AMPs in bone infection and osteomyelitis is quite limited. Here, we designed a new hydrogel that has an inhibitory effect on the proliferation of S. aureus and enhances osteogenesis. RADA16 self-assembling peptide has been applied for AMPs delivery. In this study, we demonstrated that RADA16 could form a stable structure and afford the sustained release of AMPs. The interwoven nanofiber morphology was detected by field emission scanning electron microscopy. The sustained release study revealed that the release of AMPs could be obtained until 28 days. In vitro research showed this new self-assembling hydrogel could promote the proliferation of bone mesenchymal stem cells (BMSCs) and inhibited the growth of S. aureus. More importantly, the results in vivo also proved that RADA16-AMP self-assembling peptide had an excellent effect on bone formation. Our findings implied that we successfully combined RADA16 and AMPs together and laid the foundation for the application of this new hydrogel and open new avenues for biomaterials.
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影响因子：
--
作者：
I. Marriott;Jeremy Miller;Mahnaz Sahraei
通讯作者：
I. Marriott;Jeremy Miller;Mahnaz Sahraei
RADA 自组装肽水凝胶支架中受控释放 TGF-β1
DOI：
10.2147/dddt.s109545
发表时间：
2016
期刊：
Drug design, development and therapy
影响因子：
--
作者：
Zhou A;Chen S;He B;Zhao W;Chen X;Jiang D
通讯作者：
Jiang D
影响因子：
4.9
作者：
Steinberg, DA;Hurst, MA;Fiddes, JC
通讯作者：
Fiddes, JC
DOI：
10.1073/pnas.97.12.6728
发表时间：
2000-06-06
影响因子：
11.1
作者：
Holmes, TC;de Lacalle, S;Zhang, SG
通讯作者：
Zhang, SG
影响因子：
3.7
作者：
Ni N;Hu Y;Ren H;Luo C;Li P;Wan JB;Su H
通讯作者：
Su H