Closing the Critical Period Is Required for the Maturation of Binocular Integration in Mouse Primary Visual Cortex.
Closing the Critical Period Is Required for the Maturation of Binocular Integration in Mouse Primary Visual Cortex.
复制标题
小鼠初级视觉皮层双眼整合成熟需要关闭关键期
DOI：
10.3389/fncel.2021.749265
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发表时间：
2021
影响因子：
5.3
通讯作者：
Gu Y
中科院分区：
文献类型：
--
作者：
Chan J;Hao X;Liu Q;Cang J;Gu Y
双目定向偏好匹配是双目整合的一种常见形式，被认为是立体视觉的基础。关键期可塑性是如何在正常视觉经验的指导下实现双眼匹配的，目前还没有得到充分的论证。为了研究临界期关闭如何影响双眼匹配，我们通过给予NKCC1转运蛋白拮抗剂布美他尼构建了延长临界期的小鼠模型。通过急性体内细胞外记录和分子分析，我们发现在正常的关键时期后，由于可塑性增强，同时脊柱和突触密度增加，GluA1表达上调，双眼匹配暂时中断。地西泮(DZ)/[(R， S)-3-（2-羧基哌嗪-4-基）丙基-1-膦酸（CPP）]可以弥补延长的临界期，挽救双眼匹配的缺陷。此外，延长的关键时期，仅在正常的视觉经验下，就足以完成弱视小鼠的双眼匹配。同样，通过敲掉Nogo-66受体来延长关键期至成年期也会阻碍双眼匹配和深度感知的正常成熟。这些结果表明，在青春期保持最佳的可塑性水平最有利于系统成熟。延长关键期为双眼视力的成熟提供了新的线索，并可能对弱视的治疗具有重要意义。
Binocular matching of orientation preference between the two eyes is a common form of binocular integration that is regarded as the basis for stereopsis. How critical period plasticity enables binocular matching under the guidance of normal visual experience has not been fully demonstrated. To investigate how critical period closure affects the binocular matching, a critical period prolonged mouse model was constructed through the administration of bumetanide, an NKCC1 transporter antagonist. Using acute in vivo extracellular recording and molecular assay, we revealed that binocular matching was transiently disrupted due to heightened plasticity after the normal critical period, together with an increase in the density of spines and synapses, and the upregulation of GluA1 expression. Diazepam (DZ)/[(R, S)-3-(2-carboxypiperazin-4-yl) propyl-1-phosphonic acid (CPP)] could reclose the extended critical period, and rescue the deficits in binocular matching. Furthermore, the extended critical period, alone, with normal visual experience is sufficient for the completion of binocular matching in amblyopic mice. Similarly, prolonging the critical period into adulthood by knocking out Nogo-66 receptor can prevent the normal maturation of binocular matching and depth perception. These results suggest that maintaining an optimal plasticity level during adolescence is most beneficial for the systemic maturation. Extending the critical period provides new clues for the maturation of binocular vision and may have critical implications for the treatment of amblyopia.
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影响因子：
1.9
作者：
Cang, JH;Kalatsky, VA;Stryker, MP
通讯作者：
Stryker, MP
双眼输入重合介导小鼠初级视觉皮层的关键期可塑性
影响因子：
5.3
作者：
Chen, Xiao-jing;Rasch, Malte J.;Zhang, Xiao-hui
通讯作者：
Zhang, Xiao-hui
影响因子：
3.3
作者：
Baroncelli, L.;Braschi, C.;Maffei, L.
通讯作者：
Maffei, L.
影响因子：
16.2
作者：
Chang, Jeremy T.;Whitney, David;Fitzpatrick, David
通讯作者：
Fitzpatrick, David
影响因子：
7.8
作者：
Chen, Yanqing;Nitz, Douglas A.
通讯作者：
Nitz, Douglas A.