Xenogeneic transplantation of human spermatogonia
Xenogeneic transplantation of human spermatogonia
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人精原细胞异种移植
DOI：
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发表时间：
2000
期刊：
影响因子：
--
通讯作者：
G. Palermo
中科院分区：
文献类型：
--
作者：
Marcos M. Reis;Ming C. Tsai;Peter N. Schlegel;M. Feliciano;R. Raffaelli;Zev Rosenwaks;G. Palermo
近3年来，有研究表明啮齿类动物精原细胞异种移植是可行的。使用未成熟生殖细胞注射治疗生精停滞的不育患者仅产生不良结果。我们试图建立一个完整的精子发生系的突变无精子症（W/Wv）和严重联合免疫缺陷小鼠（SCID）的睾丸移植无精子症男子的生殖细胞。生精细胞取自因阻塞性和非阻塞性无精子症而接受不育治疗的男性（平均年龄34.3 ± 9岁）的睾丸活检标本。用胶原酶消化睾丸组织以促进单个生精细胞的分离。使用10 ml注射器上的微针（内径40 μ m）将生殖细胞注射到小鼠睾丸生精小管中。为了评估细胞悬浮液渗透到小管中，使用台盼蓝作为指示剂。将小鼠饲养50至150天，以便在处死前有时间进行生殖细胞定殖和发育。然后固定睾丸进行组织学检查，并检查约100个横截面小管中的人生精细胞。从24名男性中共获得26个睾丸细胞样本，16个冷冻和10个新鲜。梗阻性（OA）16例，非梗阻性（NOA）8例。OA组生精细胞浓度为6.6 × 106个/ml，对照组为1.3 × 106个/ml。NOA组为106个/ml（p <0.01）。不同的生精细胞类型均匀分布在OA样品中，从生精细胞到完全发育的精子，但在NOA组中，大多数细胞是精原细胞和精母细胞。根据注射后立即取出的睾丸组织学切片中生精细胞的存在，成功注射了14只W/Wv小鼠的23个睾丸和12只SCID小鼠的24个睾丸。然而，注射后150天检查的剩余睾丸切片显示未发现衬有Sertoli细胞和异种生殖细胞的小管。用作受体的两种小鼠不允许植入人生殖细胞的原因可能是由于涉及不相容的细胞粘附分子和/或免疫排斥的种间特异性。
In the last 3 years, several studies have shown that xenogeneic transplantation of rodent spermatogonia is feasible. The treatment of infertile patients with spermatogenic arrest using the injection of immature germ cells has yielded only poor results. We attempted to establish a complete spermatogenetic line in the testes of mutant aspermatogenic (W/Wv) and severe combined immunodeficient mice (SCID) transplanted with germ cells from azoospermic men. Spermatogenic cells were obtained from testicular biopsy specimens of men (average age of 34.3 ± 9 years) undergoing infertility treatment because of obstructive and non-obstructive azoospermia. Testicular tissue was digested with collagenase to promote separation of individual spermatogenic cells. The germ cells were injected into mouse testicular seminiferous tubules using a microneedle (40 μm inner diameter) on a 10 ml syringe. To assess the penetration of the cell suspension into the tubules, trypan blue was used as an indicator. Mice were maintained for 50 to 150 days to allow time for germ cell colonisation and development prior to them being killed. Testes were then fixed for histological examination and approximately 100 cross-sectioned tubules were examined for human spermatogenic cells. A total of 26 testicular cell samples, 16 frozen and 10 fresh, were obtained from 24 men. The origin of the azoospermia was obstructive (OA) in 16 patients and non-obstructive (NOA) in 8 patients. The concentration of spermatogenic cells in the OA group was 6.6 × 106 cells/ml, and 1.3 ? 106 cells/ml in the NOA group (p < 0.01). The different spermatogenic cell types were distributed equally in the OA samples, ranging from spermatogenia to fully developed spermatozoa, but in the NOA group the majority of cells were spermatogonia and spermatocytes. A total of 23 testes from 14 W/Wv mice and 24 testes from 12 SCID mice were injected successfully, as judged by the presence of spermatogenic cells in histological sections of testes removed immediately after the injection. However, sections from the remaining testes examined up to 150 days after injection showed tubules lined with Sertoli cells and xenogeneic germ cells were not found. The reason why the two strains of mouse used as recipients did not allow the implantation of human germ cells is probably due to interspecies specificity involving non-compatible cell adhesion molecules and/or immunological rejection.
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影响因子：
--
作者：
L. Russell;R. Brinster
通讯作者：
L. Russell;R. Brinster
患有严重联合免疫缺陷 (scid) 的小鼠体内存在自然杀伤 (NK) 细胞。
DOI：
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发表时间：
1985
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Dorshkind,K;Pollack,SB;Bosma,MJ;Phillips,RA
通讯作者：
Phillips,RA
影响因子：
3.6
作者：
Ogawa, T;Dobrinski, I;Brinster, RL
通讯作者：
Brinster, RL
影响因子：
56.9
作者：
RUOSLAHTI, E;PIERSCHBACHER, MD
通讯作者：
PIERSCHBACHER, MD
DOI：
10.1387/ijdb.9074943
发表时间：
2003-02
期刊：
The International journal of developmental biology
影响因子：
--
作者：
T. Ogawa;J. Aréchaga;M. Avarbock;R. Brinster
通讯作者：
T. Ogawa;J. Aréchaga;M. Avarbock;R. Brinster