Unveiling structure-activity relationships of proanthocyanidins with dentin collagen.
Unveiling structure-activity relationships of proanthocyanidins with dentin collagen.
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DOI：
10.1016/j.dental.2021.08.013
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发表时间：
2021-11
期刊：
影响因子：
--
通讯作者：
Bedran-Russo AK
中科院分区：
文献类型：
--
作者：
Reis M;Zhou B;Alania Y;Leme-Kraus AA;Jing S;McAlpine JB;Chen SN;Pauli GF;Bedran-Russo AK
用16个聚合度(DP)为2~6的化学定义的原花青素(PAC)与I型胶原的构效关系(SARS)进行研究。在牙本质模型下，用动态力学分析(DMA)和红外光谱研究了PAC与I型胶原的仿生作用。用来自马尾松的PAC[单体(Mon-1和Mon-2)、二聚体(Dim-1-Dim-4)、三聚体(Tri-1-Tri-4)、四聚体(Tet-1-Tet-5)和六聚体(Hex-1)]修饰牙本质基质。采用三点弯曲浸入钳应变扫描法测定牙本质基质生物修复前后的粘弹性[储存量(E‘)、损失量(E“)、复弹性系数(E*)和tanδ]。用傅里叶变换红外光谱对牙本质基质进行生化分析。数据用单因素方差分析和组后检验进行统计分析(α=0.0 5)。DP对改良牙本质模数有显著影响(四聚体≈三聚体和六聚体≈二聚体以及单体≈对照，p<；0.001)。三聚体和四聚体使改性牙本质的力学性能提高6-8倍，并诱导胶原二级结构的构象变化。所有PAC修饰牙本质基质中均显示胶原的三级结构发生改变。研究结果确定了三个关键的SARS：(I)增加DP通常增强了PAC在调节牙本质的机械和化学性质方面的仿生潜力(Ii)牙本质胶原的二级结构受B型黄烷基间键的位置(4β→6vs.4β→8)的影响；以及(Iii)末端单体黄烷-3-醇单元在牙本质的粘弹性中起着调节作用。
To elucidate the structure-activity relationships (SARs) of proanthocyanidins (PACs) with type I collagen using sixteen chemically defined PACs with degree of polymerization (DP) 2–6. Under a dentin model, the biomimicry of PACs with type I collagen was investigated by dynamic mechanical analysis (DMA) and infrared spectroscopy. The dentin matrix was modified with PACs from Pinus massoniana [monomers (Mon-1 and Mon-2), dimers (Dim-1-Dim-4), trimers (Tri-1-Tri-4), tetramers (Tet-1-Tet-5), and hexamer (Hex-1)]. A strain sweep method in a 3-point bending submersion clamp was used to assess the viscoelastic properties [storage (E’), loss (E”), and complex moduli (E*) and tan δ] of the dentin matrix before and after biomodification. Biochemical analysis of the dentin matrix was assessed with FTIR spectroscopy. Data were statistically analyzed using one-way ANOVA and post-hoc tests (α = 0.05). DP had a significant effect on modified dentin moduli (tetramers ≈ trimers > hexamers ≈ dimers > monomers ≈ control, p < 0.001). Trimers and tetramers yielded 6- to 8-fold increase in the mechanical properties of modified dentin and induced conformational changes to the secondary structure of collagen. Modifications to the tertiary structure of collagen was shown in all PAC modified-dentin matrix. Findings establish three key SARs: (i) increasing DP generally enhances biomimicry potential of PACs in modulating the mechanical and chemical properties of dentin (ii) the secondary structure of dentin collagen is affected by the position of B-type inter-flavanyl linkages (4β→6 vs. 4β →8); and (iii) the terminal monomeric flavan-3-ol unit plays a modulatory role in the viscoelasticity of dentin.
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蛋白酶蛋白块阵列（PACBAR），用于全面捕获和描绘原蛋白素结构。
影响因子：
6.1
作者：
Jing S;Zeller WE;Ferreira D;Zhou B;Nam JW;Bedran-Russo A;Chen SN;Pauli GF
通讯作者：
Pauli GF
DOI：
10.1016/j.dental.2020.09.014
发表时间：
2020-12
期刊：
Dental materials : official publication of the Academy of Dental Materials
影响因子：
--
作者：
Alania Y;Reis MCD;Nam JW;Phansalkar RS;McAlpine J;Chen SN;Pauli GF;Bedran-Russo AK
通讯作者：
Bedran-Russo AK
影响因子：
5
作者：
Bedran-Russo, Ana K.;Pauli, Guido F.;Chen, Shao-Nong;McAlpine, James;Castellan, Carina S.;Phansalkar, Rasika S.;Aguiar, Thaiane R.;Vidal, Cristina M. P.;Napotilano, Jose G.;Nam, Joo-Won;Leme, Ariene A.
通讯作者：
Leme, Ariene A.
DOI：
10.1021/acs.joc.6b02161
发表时间：
2017-02-03
期刊：
The Journal of organic chemistry
影响因子：
--
作者：
Nam JW;Phansalkar RS;Lankin DC;McAlpine JB;Leme-Kraus AA;Vidal CM;Gan LS;Bedran-Russo A;Chen SN;Pauli GF
通讯作者：
Pauli GF
DOI：
10.1007/s10856-007-3091-9
发表时间：
2007-10-01
影响因子：
3.7
作者：
Garcia, Y.;Collighan, R.;Pandit, A.
通讯作者：
Pandit, A.