A modular hydrogel bioink containing microsphere-embedded chondrocytes for 3D-printed multiscale composite scaffolds for cartilage repair.
A modular hydrogel bioink containing microsphere-embedded chondrocytes for 3D-printed multiscale composite scaffolds for cartilage repair.
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DOI：
10.1016/j.isci.2023.107349
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发表时间：
2023-08-18
期刊：
影响因子：
5.8
通讯作者：
Huang, Wenhua
中科院分区：
文献类型：
--
作者：
Yin, Panjing;Su, Weiwei;Li, Ting;Wang, Ling;Pan, Jianying;Wu, Xiaoqi;Shao, Yan;Chen, Huabin;Lin, Lin;Yang, Yang;Cheng, Xiulin;Li, Yanbing;Wu, Yaobin;Zeng, Chun;Huang, Wenhua
关节软骨组织工程被认为是促进软骨损伤修复的替代治疗策略。在本文中，我们提出了一种包含微球嵌入软骨细胞的模块化水凝胶生物墨水，用于3D打印多尺度支架，整合了天然关节软骨的微观和宏观环境。采用微流控技术制备了明胶甲基丙烯酰（GelMA）/海藻酸钠微球，包埋于微球中的软骨细胞经冷冻复苏后仍保持活力。模块化水凝胶生物墨水可以通过凝胶中凝胶3D生物打印策略打印，用于制造多尺度水凝胶基支架。同时，细胞在支架上的增殖和分化良好，并且复合水凝胶在体内具有良好的生物相容性。这些结果表明，用于3D打印多尺度支架的含有微球嵌入的软骨细胞的模块化水凝胶生物墨水可以提供用于增强软骨修复的3D多尺度环境，考虑到关节软骨组织工程中的众多替代应用，这将是令人鼓舞的。包埋在GA-MS中的细胞即使在冷冻和复苏后仍保持活力制备含有细胞包封的GA-MS的复合水凝胶生物墨水3D打印复合水凝胶多尺度支架可以模拟天然软骨3D打印多尺度支架可以增强体内软骨修复生物材料;生物医学工程;材料科学
Articular cartilage tissue engineering is being considered an alternative treatment strategy for promoting cartilage damage repair. Herein, we proposed a modular hydrogel-based bioink containing microsphere-embedded chondrocytes for 3D printing multiscale scaffolds integrating the micro and macro environment of the native articular cartilage. Gelatin methacryloyl (GelMA)/alginate microsphere was prepared by a microfluidic approach, and the chondrocytes embedded in the microspheres remained viable after being frozen and resuscitated. The modular hydrogel bioink could be printed via the gel-in-gel 3D bioprinting strategy for fabricating the multiscale hydrogel-based scaffolds. Meanwhile, the cells cultured in the scaffolds showed good proliferation and differentiation. Furthermore, we also found that the composite hydrogel was biocompatible in vivo. These results indicated that the modular hydrogel-based bioinks containing microsphere-embedded chondrocytes for 3D printing multiscale scaffolds could provide a 3D multiscale environment for enhancing cartilage repairing, which would be encouraging considering the numerous alternative applications in articular cartilage tissue engineering. The cells embedded in GA-MS remained viable even after being frozen and resuscitated A composited hydrogel-based bioinks containing cell-encapsulated GA-MS was prepared 3D printed composited hydrogel-based multiscale scaffold can mimic native cartilage The 3D printed multiscale scaffolds could be enhancing cartilage repairing in vivo Biomaterials; Biomedical engineering; Materials science
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影响因子：
4.1
作者：
Ma C;Choi JB;Jang YS;Kim SY;Bae TS;Kim YK;Park JM;Lee MH
通讯作者：
Lee MH
DOI：
10.1016/j.jmbbm.2014.04.013
发表时间：
2014-08-01
影响因子：
3.9
作者：
Kaklamani, Georgia;Cheneler, David;Bowen, James
通讯作者：
Bowen, James
用于准确预测海藻酸盐-明胶水凝胶生物墨水的挤出印刷适性的系统热分析
影响因子：
8.4
作者：
Li Q;Zhang B;Xue Q;Zhao C;Luo Y;Zhou H;Ma L;Yang H;Bai D
通讯作者：
Bai D
影响因子：
5.5
作者：
Biondo M;Panuzzo C;Ali SM;Bozzaro S;Osella M;Bracco E;Pergolizzi B
通讯作者：
Pergolizzi B
影响因子：
9.7
作者：
Martyniak, Kari;Lokshina, Alesia;Kean, Thomas J.
通讯作者：
Kean, Thomas J.