Nanofiber scaffolds with gradients in mineral content for spatial control of osteogenesis.
Nanofiber scaffolds with gradients in mineral content for spatial control of osteogenesis.
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DOI：
10.1021/am405418g
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发表时间：
2014-02-26
影响因子：
9.5
通讯作者：
Xia, Younan
中科院分区：
文献类型：
--
作者：
Liu, Wenying;Lipner, Justin;Xie, Jingwei;Manning, Cionne N.;Thomopoulos, Stavros;Xia, Younan
关键词：
肌腱与骨的重新连接一直是骨科手术中的一个挑战。这两种组织不同的力学性能使得直接手术修复肌腱到骨的附着点变得困难。手术修复后的愈合通常不会再生自然附着，自然附着是一种复杂的组织，通过矿物质含量和细胞表型的梯度连接肌腱和骨骼。为了促进附着体的再生，我们的团队开发了一种基于纳米纤维的支架，具有分级的矿物涂层，以模拟天然肌腱到骨骼植入的矿物组成。在目前的工作中，我们评估了这种支架诱导脂肪来源的间充质干细胞(ASCs)分级成骨的能力。3-(4，5-dimethylthiazol-2-yl)-2，5-diphenyltetrazolium溴化法和增殖细胞核抗原染色结果显示，细胞增殖与矿物质含量呈负相关。相比之下，碱性磷酸酶染色是成骨的一个指标，与矿物质含量呈正相关。同样，矮小相关转录因子2(成骨细胞分化的早期标志)和骨钙素(成骨细胞分化的晚期标志)免疫染色均与矿物质含量呈正相关。这些结果表明，纳米纤维支架表面矿物质含量的梯度能够诱导ASCs逐步分化为成骨细胞，用于成牙本质修复。
Reattachment of tendon to bone has been a challenge in orthopedic surgery. The disparate mechanical properties of the two tissues make it difficult to achieve direct surgical repair of the tendon-to-bone insertion. Healing after surgical repair typically does not regenerate the natural attachment, a complex tissue that connects tendon and bone across a gradient in both mineral content and cell phenotypes. To facilitate the regeneration of the attachment, our groups have developed a nanofiber-based scaffold with a graded mineral coating to mimic the mineral composition of the native tendon-to-bone insertion. In the present work, we evaluated the ability of this scaffold to induce graded osteogenesis of adipose-derived mesenchymal stem cells (ASCs). Results from 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay and proliferating cell nuclear antigen staining indicated that cell proliferation was negatively correlated with the mineral content. In contrast, alkaline phosphatase staining, an indicator of osteogenesis, was positively correlated with the mineral content. Likewise, runt-related transcription factor 2 (an early marker of osteoblast differentiation) and osteocalcin (a late marker of osteoblast differentiation) immunostaining were both positively correlated with the mineral content. These results indicate that a gradient in mineral content on the surface of a nanofiber scaffold is capable of inducing graded differentiation of ASCs into osteoblasts for enthesis repair.
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影响因子：
14
作者：
Kim, SS;Park, MS;Kim, BS
通讯作者：
Kim, BS
影响因子：
64.5
作者：
Lee, Na Kyung;Sowa, Hideaki;Karsenty, Gerard
通讯作者：
Karsenty, Gerard
Osf2/Cbfa1：成骨细胞分化的转录激活因子
影响因子：
64.5
作者：
Ducy, P;Zhang, R;Karsenty, G
通讯作者：
Karsenty, G
DOI：
10.1007/978-1-4419-1050-9_5
发表时间：
2010-01-01
期刊：
OSTEOIMMUNOLOGY: INTERACTIONS OF THE IMMUNE AND SKELETAL SYSTEMS II
影响因子：
--
作者：
Komori, Toshihisa
通讯作者：
Komori, Toshihisa
影响因子：
4.1
作者：
Meleti, Z;Shapiro, IM;Adams, CS
通讯作者：
Adams, CS