Ciprofloxacin-loaded bioadhesive hydrogels for ocular applications.
Ciprofloxacin-loaded bioadhesive hydrogels for ocular applications.
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DOI：
10.1039/d0bm00935k
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发表时间：
2020-09-15
影响因子：
6.6
通讯作者：
Annabi N
中科院分区：
文献类型：
--
作者：
Khalil IA;Saleh B;Ibrahim DM;Jumelle C;Yung A;Dana R;Annabi N
严重角膜感染的治疗通常需要复杂的治疗方案，包括长期和高频使用抗生素，这给患者带来了许多挑战，并影响了治疗方案的依从性。在导致组织缺陷的严重损伤（例如角膜撕裂）的情况下，局部药物治疗方案是不够的，通常需要缝合。因此，对能够在预防或治疗感染的同时提供组织闭合的干预措施的需求尚未得到满足。在这项研究中，我们描述了一种抗菌生物胶粘剂水凝胶的开发，该水凝胶负载含有环丙沙星（CPX）的胶束，用于治疗有感染风险的角膜损伤。体外释放谱显示，该水凝胶体系可释放广谱抗菌药物CPX达24h。此外，所开发的cpx负载水凝胶对金黄色葡萄球菌和铜绿假单胞菌这两种主要引起眼部感染的细菌具有优异的抗菌性能。通过物理表征、粘附和细胞相容性测试来评估CPX在制备的水凝胶中的作用。结果表明，CPX负载不影响水凝胶的硬度、粘附性能或细胞相容性。用感染性猪角膜损伤离体模型评价抗菌水凝胶的效果。与未处理和未使用CPX的水凝胶处理的角膜组织相比，经抗菌水凝胶处理的角膜组织在24小时后细菌集落形成单位（CFU）显著降低，角膜上皮活力更高。这些结果表明，所开发的黏附水凝胶系统提供了一种有前途的无缝线解决方案，可以密封角膜伤口，同时防止感染。胶束负载在光交联明胶基水凝胶中，作为一种无创无缝线方法用于角膜组织损伤感染后的再生
The management of serious corneal infections often requires complex therapeutic regimens involving the prolonged and high-frequency application of antibiotics that provide many challenges to patients and impact compliance with the therapeutic regimens. In the context of severe injuries that lead to tissue defects (e.g. corneal lacerations) topical drug regimens are inadequate and suturing is often indicated. There is thus an unmet need for interventions that can provide tissue closure while concurrently preventing or treating infection. In this study, we describe the development of an antibacterial bioadhesive hydrogel loaded with micelles containing ciprofloxacin (CPX) for the management of corneal injuries at risk of infection. The in vitro release profile showed that the hydrogel system can release CPX, a broad-spectrum antibacterial drug, for up to 24h. Moreover, the developed CPX-loaded hydrogels exhibited excellent antibacterial properties against Staphylococcus aureus and Pseudomonas aeruginosa, two bacterial strains responsible for most ocular infections. Physical characterization, as well as adhesion and cytocompatibility tests, were performed to assess the effect of CPX loading in the developed hydrogel. Results showed that CPX loading did not affect stiffness, adhesive properties, or cytocompatibility of hydrogels. The efficiency of the antibacterial hydrogel was assessed using an ex vivo model of infectious pig corneal injury. Corneal tissues treated with the antibacterial hydrogel showed a significant decrease in bacterial colony-forming units (CFU) and a higher corneal epithelial viability after 24h as compared to non-treated corneas and corneas treated with hydrogel without CPX. These results suggest that the developed adhesive hydrogel system presents a promising suture-free solution to seal corneal wounds while preventing infections. Micelles loaded in a photocrosslinkable gelatin based hydrogel as a non-invasive suture-free approach for corneal tissue regeneration after injury with infection
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影响因子：
6.6
作者：
Champeau, Mathilde;Thomassin, Jean-Michel;Jerome, Christine
通讯作者：
Jerome, Christine
影响因子：
5.8
作者：
Budai, Livia;Hajdu, Maria;Antal, Istvan
通讯作者：
Antal, Istvan
DOI：
10.1016/j.ejpb.2010.02.011
发表时间：
2010-06-01
影响因子：
4.9
作者：
Gratieri, Tais;Gelfuso, Guilherme Martins;Vianna Lopez, Renata Fonseca
通讯作者：
Vianna Lopez, Renata Fonseca
影响因子：
2
作者：
Gupta, Raghav;Patil, Bharat;Dada, Tanuj
通讯作者：
Dada, Tanuj
DOI：
10.1016/j.ijbiomac.2014.09.020
发表时间：
2015-01-01
影响因子：
8.2
作者：
Islan, German A.;Mukherjee, Arup;Castro, Guillermo R.
通讯作者：
Castro, Guillermo R.