Improving the Intercellular Uptake and Osteogenic Potency of Calcium Phosphate via Nanocomplexation with the RALA Peptide.
Improving the Intercellular Uptake and Osteogenic Potency of Calcium Phosphate via Nanocomplexation with the RALA Peptide.
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DOI：
10.3390/nano10122442
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发表时间：
2020-12-07
期刊：
影响因子：
--
通讯作者：
McCarthy HO
中科院分区：
文献类型：
--
作者：
O'Doherty M;Mulholland EJ;Chambers P;Pentlavalli S;Ziminska M;Chalanqui MJ;Pauly HM;Sathy BN;Donahue TH;Kelly DJ;Dunne N;McCarthy HO
磷酸钙基材料（例如，α-磷酸三钙（α-TCP））已显示促进干/祖细胞的成骨分化、增强成骨细胞的成骨活性并介导体内骨组织形成。然而，磷酸钙的可变粒度和亲水性导致生物利用度极低。因此，需要一种有效的递送系统，其可以包封磷酸钙，改善细胞进入，并因此在成骨细胞中引起有效的成骨反应。在这项研究中，评估了成骨细胞系细胞的胶原基质沉积和细胞外基质矿化，以研究α-TCP纳米颗粒细胞内递送后的成骨作用。通过与新型阳离子30聚体两亲肽（RALA）缩合形成纳米颗粒。以5：1的质量比制备的纳米颗粒表现出43 nm的平均粒径和+26 mV的ζ电位。平均粒度和zeta电位在室温下和在一系列温度（4 - 37 ° C）下保持稳定长达28天。在RALA/α-TCP纳米颗粒处理后转染后24小时细胞活力降低;然而，到第7天恢复。在MG-63细胞中用RALA/α-TCP纳米颗粒（NP）转染后第21天，I型胶原的免疫细胞化学染色显示与未处理的对照相比胶原表达和沉积显著增强。此外，在猪间充质干细胞（pMSC）中，与单独的α-TCP相比，矿化增强。综上所述，这些数据表明RALA/α-TCP NP的内化在MG-63和pMSC两者中均引起有效的成骨应答。
Calcium phosphate-base materials (e.g., alpha tri-calcium phosphate (α–TCP)) have been shown to promote osteogenic differentiation of stem/progenitor cells, enhance osteoblast osteogenic activity and mediate in vivo bone tissue formation. However, variable particle size and hydrophilicity of the calcium phosphate result in an extremely low bioavailability. Therefore, an effective delivery system is required that can encapsulate the calcium phosphate, improve cellular entry and, consequently, elicit a potent osteogenic response in osteoblasts. In this study, collagenous matrix deposition and extracellular matrix mineralization of osteoblast lineage cells were assessed to investigate osteogenesis following intracellular delivery of α-TCP nanoparticles. The nanoparticles were formed via condensation with a novel, cationic 30 mer amphipathic peptide (RALA). Nanoparticles prepared at a mass ratio of 5:1 demonstrated an average particle size of 43 nm with a zeta potential of +26 mV. The average particle size and zeta potential remained stable for up to 28 days at room temperature and across a range of temperatures (4–37 °C). Cell viability decreased 24 h post-transfection following RALA/α-TCP nanoparticle treatment; however, recovery ensued by Day 7. Immunocytochemistry staining for Type I collagen up to Day 21 post-transfection with RALA/α-TCP nanoparticles (NPs) in MG-63 cells exhibited a significant enhancement in collagen expression and deposition compared to an untreated control. Furthermore, in porcine mesenchymal stem cells (pMSCs), there was enhanced mineralization compared to α–TCP alone. Taken together these data demonstrate that internalization of RALA/α-TCP NPs elicits a potent osteogenic response in both MG-63 and pMSCs.
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影响因子：
12.7
作者：
Dang M;Saunders L;Niu X;Fan Y;Ma PX
通讯作者：
Ma PX
DOI：
10.1073/pnas.1208916109
发表时间：
2012-08-28
影响因子：
11.1
作者：
Boonrungsiman, Suwimon;Gentleman, Eileen;Stevens, Molly M.
通讯作者：
Stevens, Molly M.
影响因子：
15.9
作者：
Hu, Kai;Olsen, Bjorn R.
通讯作者：
Olsen, Bjorn R.
DOI：
10.1007/978-1-4419-1050-9_5
发表时间：
2010-01-01
期刊：
OSTEOIMMUNOLOGY: INTERACTIONS OF THE IMMUNE AND SKELETAL SYSTEMS II
影响因子：
--
作者：
Komori, Toshihisa
通讯作者：
Komori, Toshihisa
影响因子：
3.3
作者：
Dunfield, D;Sayer, M;Shurvell, HF
通讯作者：
Shurvell, HF