Reconfigurable microfluidic dilution for high-throughput quantitative assays.
Reconfigurable microfluidic dilution for high-throughput quantitative assays.
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DOI：
10.1039/c5lc00432b
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发表时间：
2015-06-21
期刊：
影响因子：
6.1
通讯作者：
Pan T
中科院分区：
文献类型：
--
作者：
Fan J;Li B;Xing S;Pan T
本文报道了一种用于高通量定量分析的可重构微流体稀释装置，其可以容易地从固定的样品体积（例如，10 μL），而无需连续流体泵或机器人自动化的任何辅助。集成的稀释生成芯片由可切换的分配和收集通道、计量容器、反应室和压力激活的拉普拉斯阀组成。在将样品、稀释剂和检测试剂顺序装载到各个计量室中之后，顶部微流体层可以被重新配置以将计量的化学品并行地收集到反应室中，在反应室中将进行检测。为了促进微腔中的混合和反应，已经研究了两种声微流致动机制，以便于集成和可访问性。此外，微流体稀释发生器已通过比色和荧光手段表征。定量稀释芯片的通用用途的进一步证明利用了通常可用的二喹啉甲酸（BCA）测定来分析人体组织提取物的蛋白质浓度。简而言之，微流控稀释发生器通过在具有最小设备要求的紧凑微流控装置中简单操纵微量化学品，提供了常规定量测定平台的高通量高效定量分析替代方案，其可以用作常规实验室、护理点和低资源环境中的生化和生物分析的简易工具。
This paper reports a reconfigurable microfluidic dilution device for high-throughput quantitative assays, which can easily produce discrete logarithmic/binary concentration profiles ranging from 1 to 100-fold dilution in parallel from a fixed sample volume (e.g., 10 μL) without any assistance of continuous fluidic pump or robotic automation. The integrated dilution generation chip consists of switchable distribution and collection channels, metering reservoirs, reaction chambers, and pressure-activatable Laplace valves. Following the sequential loading of a sample, a diluent, and a detection reagent into the individual metering chambers, the top microfluidic layer can be reconfigured to collect the metered chemicals into the reaction chambers in parallel, where detection will be conducted. To facilitate mixing and reaction in the micro-chambers, two acoustic microstreaming actuation mechanisms have been investigated for easy integratability and accessibility. Furthermore, the microfluidic dilution generator has been characterized by both colorimetric and fluorescent means. A further demonstration of the generic usage of the quantitative dilution chip has utilized a commonly available bicinchoninic acid (BCA) assay to analyse protein concentrations of human tissue extracts. In brief, the microfluidic dilution generator offers a high-throughput high-efficiency quantitative analytical alternative to the conventional quantitative assay platforms, by simple manipulation of a minute amount of chemicals in a compact microfluidic device with a minimal equipment requirement, which can serve as a facile tool for biochemical and biological analyses in regular laboratories, point-of-care and low-resource environments.
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