High-fidelity tissue engineering of patient-specific auricles for reconstruction of pediatric microtia and other auricular deformities.
High-fidelity tissue engineering of patient-specific auricles for reconstruction of pediatric microtia and other auricular deformities.
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DOI：
10.1371/journal.pone.0056506
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Spector JA
中科院分区：
文献类型：
--
作者：
Reiffel AJ;Kafka C;Hernandez KA;Popa S;Perez JL;Zhou S;Pramanik S;Brown BN;Ryu WS;Bonassar LJ;Spector JA
自体技术用于儿童小体畸形的重建往往导致不理想的美学结果和发病率在肋软骨供体部位。因此，我们寻求将数字摄影测量与CAD/CAM技术相结合，开发I型胶原水凝胶支架及其相应的模具，以精确模拟患者特定外耳的正常解剖结构，并再现天然耳廓弹性软骨的复杂生物力学特性，同时避免传统自体重建的发病率。将正常儿童耳的三维结构数字化并转换为虚拟实体进行模具设计。这些耳朵的基于图像的合成重建是由I型胶原水凝胶制成的。一半用牛耳软骨细胞播种。在裸鼠背部皮下植入细胞和非细胞构建物，分别于1和3个月后收获。肉眼检查显示，1个月后，无细胞植入物的大小明显减小。即使在3个月后，细胞结构仍然保持了动物背部的轮廓/投影。收获后1个月和3个月，细胞构建体的重量显著大于非细胞构建体。红素o染色显示细胞结构显示出自组装的软骨膜层和丰富的新软骨沉积的证据。1个月细胞构建的Verhoeff染色显示新生弹性软骨沉积，3个月后更为广泛和坚固。3个月后，细胞构建物的平衡模量和水力渗透性与天然牛耳软骨无显著差异。我们已经开发了高保真度、生物相容性、患者特异性的组织工程耳廓重建构建体，即使在长时间植入后，也能在生物力学和组织学上很大程度上模仿天然耳廓。这种策略在未来具有巨大的潜力，用于持久的患者特异性组织工程解剖适当的耳廓重建。
Autologous techniques for the reconstruction of pediatric microtia often result in suboptimal aesthetic outcomes and morbidity at the costal cartilage donor site. We therefore sought to combine digital photogrammetry with CAD/CAM techniques to develop collagen type I hydrogel scaffolds and their respective molds that would precisely mimic the normal anatomy of the patient-specific external ear as well as recapitulate the complex biomechanical properties of native auricular elastic cartilage while avoiding the morbidity of traditional autologous reconstructions. Three-dimensional structures of normal pediatric ears were digitized and converted to virtual solids for mold design. Image-based synthetic reconstructions of these ears were fabricated from collagen type I hydrogels. Half were seeded with bovine auricular chondrocytes. Cellular and acellular constructs were implanted subcutaneously in the dorsa of nude rats and harvested after 1 and 3 months. Gross inspection revealed that acellular implants had significantly decreased in size by 1 month. Cellular constructs retained their contour/projection from the animals' dorsa, even after 3 months. Post-harvest weight of cellular constructs was significantly greater than that of acellular constructs after 1 and 3 months. Safranin O-staining revealed that cellular constructs demonstrated evidence of a self-assembled perichondrial layer and copious neocartilage deposition. Verhoeff staining of 1 month cellular constructs revealed de novo elastic cartilage deposition, which was even more extensive and robust after 3 months. The equilibrium modulus and hydraulic permeability of cellular constructs were not significantly different from native bovine auricular cartilage after 3 months. We have developed high-fidelity, biocompatible, patient-specific tissue-engineered constructs for auricular reconstruction which largely mimic the native auricle both biomechanically and histologically, even after an extended period of implantation. This strategy holds immense potential for durable patient-specific tissue-engineered anatomically proper auricular reconstructions in the future.
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影响因子：
3.1
作者：
Gleghorn, J. P.;Jones, A. R. C.;Bonassar, L. J.
通讯作者：
Bonassar, L. J.
影响因子：
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作者：
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通讯作者：
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影响因子：
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作者：
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通讯作者：
Vacanti, CA
影响因子：
3.6
作者：
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通讯作者：
Lequerica, Juan L.
影响因子：
1.5
作者：
Romo, Thomas, III;Morris, Luc G. T.;Kohan, Darius
通讯作者：
Kohan, Darius