Genomic characterization of Bordetella pertussis in South Africa, 2015-2019.
Genomic characterization of Bordetella pertussis in South Africa, 2015-2019.
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DOI：
10.1099/mgen.0.001162
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发表时间：
2023-12
影响因子：
3.9
通讯作者：
Wolter, Nicole
中科院分区：
文献类型：
--
作者：
Moosa, Fahima;du Plessis, Mignon;Weigand, Michael R.;Peng, Yanhui;Mogale, Dineo;de Gouveia, Linda;Nunes, Marta C.;Madhi, Shabir A.;Zar, Heather J.;Reubenson, Gary;Ismail, Arshad;Tondella, M. Lucia;Cohen, Cheryl;Walaza, Sibongile;von Gottberg, Anne;Wolter, Nicole
在南非，百日咳仍然是一个公共卫生问题，近年来报告的病例和暴发有所增加。2015至2019年间，对来自南非三个不同监测项目的32株百日咳杆菌进行了全基因组测序。用多位点序列分型、疫苗抗原基因(ptxp、ptxA、ptxB、prn和fimH)和全基因组结构分析基因组序列。所有分离株均为序列类型2，含有百日咳毒素启动子等位基因ptxP3。优势基因为ptxP3-ptxA1-ptxB2-prn2-fimH2(31/32，96.9 %)，未发现疫苗抗原基因的Pertactin缺陷或其他突变。在21个产生封闭基因组组合的菌株中，检测到8个不同的基因组结构，其中61.9%(13/21)的菌株具有3个优势结构。病例数量的增加可能不是由于基因组的进化变化，而可能是由于其他因素，如百日咳杆菌病的周期性、使用无细胞疫苗导致的免疫力减弱和/或群体免疫缺口。
Pertussis remains a public health concern in South Africa, with an increase in reported cases and outbreaks in recent years. Whole genome sequencing was performed on 32 Bordetella pertussis isolates sourced from three different surveillance programmes in South Africa between 2015 and 2019. Genome sequences were characterized using multilocus sequence typing, vaccine antigen genes (ptxP, ptxA, ptxB, prn and fimH) and overall genome structure. All isolates were sequence type 2 and harboured the pertussis toxin promoter allele ptxP3. The dominant genotype was ptxP3-ptxA1-ptxB2-prn2-fimH2 (31/32, 96.9 %), with no pertactin-deficient or other mutations in vaccine antigen genes identified. Amongst 21 isolates yielding closed genome assemblies, eight distinct genome structures were detected, with 61.9 % (13/21) of the isolates exhibiting three predominant structures. Increases in case numbers are probably not due to evolutionary changes in the genome but possibly due to other factors such as the cyclical nature of B. pertussis disease, waning immunity due to the use of acellular vaccines and/or population immunity gaps.
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DOI：
10.1007/s10096-014-2297-2
发表时间：
2015-04
影响因子：
4.5
作者：
van Gent, M.;Heuvelman, C. J.;van der Heide, H. G.;Hallander, H. O.;Advani, A.;Guiso, N.;von Konig, C. H. Wirsing;Vestrheim, D. F.;Dalby, T.;Fry, N. K.;Pierard, D.;Detemmerman, L.;Zavadilova, J.;Fabianova, K.;Logan, C.;Habington, A.;Byrne, M.;Lutynska, A.;Mosiej, E.;Pelaz, C.;Grondahl-Yli-Hannuksela, K.;Barkoff, A. M.;Mertsola, J.;Economopoulou, A.;He, Q.;Mooi, F. R.
通讯作者：
Mooi, F. R.
影响因子：
4.5
作者：
Fisman, David N.;Tang, Patrick;Jamieson, Frances
通讯作者：
Jamieson, Frances
影响因子：
11.8
作者：
Bouchez V;Guglielmini J;Dazas M;Landier A;Toubiana J;Guillot S;Criscuolo A;Brisse S
通讯作者：
Brisse S
影响因子：
3.9
作者：
Ring N;Davies H;Morgan J;Sundaresan M;Tiong A;Preston A;Bagby S
通讯作者：
Bagby S
影响因子：
3.7
作者：
Moosa, Fahima;du Plessis, Mignon;von Gottberg, Anne
通讯作者：
von Gottberg, Anne