Analysis of Bordetella pertussis clinical isolates circulating in European countries during the period 1998-2012.
Analysis of Bordetella pertussis clinical isolates circulating in European countries during the period 1998-2012.
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DOI：
10.1007/s10096-014-2297-2
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发表时间：
2015-04
影响因子：
4.5
通讯作者：
Mooi, F. R.
中科院分区：
文献类型：
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作者：
van Gent, M.;Heuvelman, C. J.;van der Heide, H. G.;Hallander, H. O.;Advani, A.;Guiso, N.;von Konig, C. H. Wirsing;Vestrheim, D. F.;Dalby, T.;Fry, N. K.;Pierard, D.;Detemmerman, L.;Zavadilova, J.;Fabianova, K.;Logan, C.;Habington, A.;Byrne, M.;Lutynska, A.;Mosiej, E.;Pelaz, C.;Grondahl-Yli-Hannuksela, K.;Barkoff, A. M.;Mertsola, J.;Economopoulou, A.;He, Q.;Mooi, F. R.
尽管接种了50多年的疫苗，百日咳仍然是一种地方病，经常爆发疫情。除波兰外，欧洲国家在20世纪90年代已经用无细胞疫苗(ACV)取代了全细胞疫苗(WCVS)。在世界范围内，疫苗株和流行株之间在疫苗抗原上存在着抗原差异。本研究对1998-2012年间从欧洲13个国家分离到的466株百日咳杆菌进行了百日咳毒素启动子(PTXP)多位点抗原序列分型(MAST)和ACV蛋白编码基因的多位点分型：百日咳毒素(PTX)、百日咳蛋白(PRN)、2型菌毛(Fim2)和3型菌毛(Fim3)。进一步对分离株进行菌毛分型、多位点变数串联重复序列分析(MLVA)和脉冲场凝胶电泳(PFGE)。结果显示，使用ACV的12个国家的百日咳杆菌种群非常相似，而使用WCV的波兰则非常不同，这表明ACV和WCVS选择不同的百日咳杆菌种群。这项研究为今后研究疫苗接种方案对百日咳杆菌种群的影响奠定了基线。本文的在线版本(doi：10.1007/s10096-0142297-2)包含补充材料，授权用户可以使用。
Despite more than 50 years of vaccination, pertussis is still an endemic disease, with regular epidemic outbreaks. With the exception of Poland, European countries have replaced whole-cell vaccines (WCVs) by acellular vaccines (ACVs) in the 1990s. Worldwide, antigenic divergence in vaccine antigens has been found between vaccine strains and circulating strains. In this work, 466 Bordetella pertussis isolates collected in the period 1998–2012 from 13 European countries were characterised by multi-locus antigen sequence typing (MAST) of the pertussis toxin promoter (ptxP) and of the genes coding for proteins used in the ACVs: pertussis toxin (Ptx), pertactin (Prn), type 2 fimbriae (Fim2) and type 3 fimbriae (Fim3). Isolates were further characterised by fimbrial serotyping, multi-locus variable-number tandem repeat analysis (MLVA) and pulsed-field gel electrophoresis (PFGE). The results showed a very similar B. pertussis population for 12 countries using ACVs, while Poland, which uses a WCV, was quite distinct, suggesting that ACVs and WCVs select for different B. pertussis populations. This study forms a baseline for future studies on the effect of vaccination programmes on B. pertussis populations. The online version of this article (doi:10.1007/s10096-014-2297-2) contains supplementary material, which is available to authorized users.
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影响因子：
9.4
作者：
Advani, Abdolreza;Hallander, Hans O.;He, Qiushui
通讯作者：
He, Qiushui
影响因子：
8
作者：
Cherry, JD
通讯作者：
Cherry, JD
影响因子：
5.5
作者：
Dakic, Gordana;Kallonen, Teemu;He, Qiushui
通讯作者：
He, Qiushui
影响因子：
--
作者：
Borisova, Olga;Kombarova, Svetlana Yu;Mooi, Frits R.
通讯作者：
Mooi, Frits R.
影响因子：
11.8
作者：
Lam C;Octavia S;Ricafort L;Sintchenko V;Gilbert GL;Wood N;McIntyre P;Marshall H;Guiso N;Keil AD;Lawrence A;Robson J;Hogg G;Lan R
通讯作者：
Lan R