Circular RNA RBPMS inhibits bladder cancer progression via miR-330-3p/RAI2 regulation.
Circular RNA RBPMS inhibits bladder cancer progression via miR-330-3p/RAI2 regulation.
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DOI：
10.1016/j.omtn.2021.01.009
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发表时间：
2021-03-05
期刊：
影响因子：
--
通讯作者：
Jiang H
中科院分区：
文献类型：
--
作者：
Yang C;Mou Z;Zhang Z;Wu S;Zhou Q;Chen Y;Gong J;Xu C;Ou Y;Chen X;Dai X;Jiang H
膀胱癌是一种严重的恶性肿瘤，因其侵袭和转移而死亡率高。越来越多的证据表明，环状rna在生物学功能中起着至关重要的作用，与膀胱癌的增殖和侵袭密切相关。在我们的研究中，我们采用qRT-PCR、RNA荧光原位杂交（FISH）、5-乙基-2 ' -脱氧尿苷（EdU）、CCK-8、Transwell测定、荧光素酶报告基因测定、异种移植和实时成像来检测环状RNA结合蛋白与多重剪接（circRBPMS）在膀胱癌（BC）中的作用。采用生物信息学分析和WB方法探讨其调控机制。环状rna （circRNAs）在BC中的表达谱分析显示，circRBPMS显著下调。低circRBPMS表达与侵袭性BC表型相关，而circRBPMS的上调通过直接靶向miR-330-3p/维甲酸诱导2 （RAI2）轴抑制BC细胞增殖和转移。miR-330-3p上调或沉默RAI2可恢复circRBPMS过表达后的BC细胞增殖、侵袭和迁移。在体外，RAI2沉默逆转了mir -330-3p诱导的细胞侵袭和迁移以及生长抑制。此外，通过对TCGA数据库中RAI2下游靶点的生物信息学分析，我们通过RAI2介导的ERK和上皮间质转化（epithelial-mesenchymal transition， EMT）途径确定并验证了circRBPMS的生物学作用。我们总结了circRBPMS/miR-330-3p/RAI2轴，其中circRBPMS作为肿瘤抑制因子，并为BC提供了潜在的生物标志物和治疗靶点。Yang等证实circRBPMS可通过circRBPMS/miR-330-3p / RAI2调控抑制BC的发生发展，为BC的治疗提供了新的诊疗靶点。
Bladder cancer is a severe cancer with high mortality because of invasion and metastasis. Growing evidence has revealed that circular RNAs play critical roles in biological function, which is closely connected to proliferation and invasion of bladder cancer. In our study, we employed qRT-PCR, RNA fluorescence in situ hybridization (FISH), 5-ethynyl-2′-deoxyuridine (EdU), CCK-8, Transwell assays, luciferase reporter assays, xenografts, and live imaging to detect the roles of circular RNA binding protein with multiple splicing (circRBPMS) in bladder cancer (BC). Bioinformatics analysis and WB were performed to investigate the regulatory mechanism. Expression profile analysis of circular RNAs (circRNAs) in BC revealed that circRBPMS was significantly downregulated. Low circRBPMS expression correlates with aggressive BC phenotypes, whereas upregulation of circRBPMS suppresses BC cell proliferation and metastasis by directly targeting the miR-330-3p/ retinoic acid induced 2 (RAI2) axis. miR-330-3p upregulation or silencing of RAI2 restored BC cell proliferation, invasion, and migration following overexpression of circRBPMS. RAI2 silencing reversed miR-330-3p-induced cell invasion and migration as well as growth inhibition in vitro. Moreover, through bioinformatic analysis of the downstream target of RAI2 in the TCGA database, we identified and validated the biological role of circRBPMS through the RAI2-mediated ERK and epithelial-mesenchymal transition (EMT) pathways. We summarize the circRBPMS/miR-330-3p/RAI2 axis, where circRBPMS acts as a tumor suppressor, and provide a potential biomarker and therapeutic target for BC. Yang et al. confirm that circRBPMS can inhibit the occurrence and development of BC through circRBPMS/miR-330-3p / RAI2 regulation, which provides a new diagnostic and therapeutic target for BC treatment.
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影响因子：
28.2
作者：
Esposito M;Kang Y
通讯作者：
Kang Y
DOI：
10.3322/caac.21244
发表时间：
2014-09
期刊：
CA: a cancer journal for clinicians
影响因子：
--
作者：
Berindan-Neagoe I;Monroig Pdel C;Pasculli B;Calin GA
通讯作者：
Calin GA
影响因子：
2.9
作者：
Jin, Zhe;Jia, Baoxing;Liu, Yahui
通讯作者：
Liu, Yahui
侵袭相关环状RNA circFNDC3B通过miR-1178-3p/G3BP2/SRC/FAK轴抑制膀胱癌进展
影响因子：
37.3
作者：
Liu H;Bi J;Dong W;Yang M;Shi J;Jiang N;Lin T;Huang J
通讯作者：
Huang J
影响因子：
46.9
作者：
Jeck, William R.;Sharpless, Norman E.
通讯作者：
Sharpless, Norman E.