Development of a standardized histopathology scoring system for intervertebral disc degeneration and regeneration in rabbit models-An initiative of the ORSspine section.
Development of a standardized histopathology scoring system for intervertebral disc degeneration and regeneration in rabbit models-An initiative of the ORSspine section.
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DOI：
10.1002/jsp2.1147
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发表时间：
2021-06
期刊：
影响因子：
3.7
通讯作者：
Iatridis JC
中科院分区：
文献类型：
--
作者：
Gullbrand SE;Ashinsky BG;Lai A;Gansau J;Crowley J;Cunha C;Engiles JB;Fusellier M;Muehleman C;Pelletier M;Presciutti S;Schol J;Takeoka Y;Yurube T;Zhang Y;Masuda K;Iatridis JC
兔腰椎是研究椎间盘退变和再生疗法临床前评估的常用模型。组织病理学是评估退变、修复或治疗后椎间盘形态和细胞性改变的基础。尽管如此，对于任何常用的动物模型，脊柱领域还没有建立标准化的组织学分级标准。本研究的目的是建立一种新的标准化评分系统来评估兔模型的椎间盘退变和再生。评分系统是在回顾文献和对脊柱研究人员进行调查后制定的。使用由4个独立实验室提供的图像对评分系统进行验证，这些图像由12名不同经验水平的独立评分员进行评分。信度检验是通过计算每个类别的组内相关系数(ICC)和总分来进行的。然后，根据国际商会的分析和作者之间的讨论结果，进一步完善了评分系统。最终的总评分系统包括从0(健康)到2(严重退变)的7个特征(髓核形状、面积、细胞和基质凝聚、纤维环/髓核边缘形态、纤维环形态和终板硬化/增厚)的评分。ICCS总体上在评分者中表现出中等到良好的一致性。还包括一份主要评分系统的附录，用于评估再生疗法的研究，该研究涉及对髓核和纤维内环内的细胞克隆和形态进行评分。总体而言，这一新的评分系统提供了一条提高标准化的途径，允许在实验室之间进行更准确的比较，并对整个领域的病理生理学和再生治疗进行更有力的评估。本研究根据文献报道的实践、对脊柱研究人员的调查和验证测试，开发了兔模型退变和再生的组织病理学评分系统。兔腰椎是研究椎间盘退变和再生治疗的临床前评估的常用模型，组织病理学是评估退变或修复或治疗后椎间盘形态和细胞性变化的基础。尽管如此，对于任何常用的动物模型，脊柱领域还没有建立标准化的组织学分级标准。因此，本研究的目的是建立一种新的标准化评分系统来评估兔模型的椎间盘退变和再生。
The rabbit lumbar spine is a commonly utilized model for studying intervertebral disc degeneration and for the pre‐clinical evaluation of regenerative therapies. Histopathology is the foundation for which alterations to disc morphology and cellularity with degeneration, or following repair or treatment are assessed. Despite this, no standardized histology grading scale has yet been established for the spine field for any of the frequently utilized animal models. The purpose of this study was to establish a new standardized scoring system to assess disc degeneration and regeneration in the rabbit model. The scoring system was formulated following a review of the literature and a survey of spine researchers. Validation of the scoring system was carried out using images provided by 4 independent laboratories, which were graded by 12 independent graders of varying experience levels. Reliability testing was performed via the computation of intra‐class correlation coefficients (ICC) for each category and the total score. The scoring system was then further refined based on the results of the ICC analysis and discussions amongst the authors. The final general scoring system involves scoring 7 features (nucleus pulposus shape, area, cellularity and matrix condensation, annulus fibrosus/nucleus pulposus border appearance, annulus fibrosus morphology, and endplate sclerosis/thickening) on a 0 (healthy) to 2 (severe degeneration) scale. ICCs demonstrated overall moderate to good agreement across graders. An addendum to the main scoring system is also included for use in studies evaluating regenerative therapeutics, which involves scoring cell cloning and morphology within the nucleus pulposus and inner annulus fibrosus. Overall, this new scoring system provides an avenue to improve standardization, allow a more accurate comparison between labs and more robust evaluation of pathophysiology and regenerative treatments across the field. This study developed a histopathology scoring system for degeneration and regeneration in the rabbit model based on reported practice in the literature, a survey of spine researchers, and validation testing. The rabbit lumbar spine is a commonly utilized model for studying intervertebral disc degeneration and for the pre‐clinical evaluation of regenerative therapies, and histopathology is the foundation for which alterations to disc morphology and cellularity with degeneration, or following repair or treatment are assessed. Despite this, no standardized histology grading scale has yet been established for the spine field for any of the frequently utilized animal models. Therefore, the purpose of this study was to establish a new standardized scoring system to assess disc degeneration and regeneration in the rabbit model.
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影响因子：
3
作者：
THOMPSON, JP;PEARCE, RH;BISHOP, PB
通讯作者：
BISHOP, PB
影响因子：
3
作者：
ROBERTS, S;MENAGE, J;URBAN, JPG
通讯作者：
URBAN, JPG
DOI：
10.1002/jbmr.4009
发表时间：
2020-08
期刊：
Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research
影响因子：
--
作者：
Ashinsky BG;Bonnevie ED;Mandalapu SA;Pickup S;Wang C;Han L;Mauck RL;Smith HE;Gullbrand SE
通讯作者：
Gullbrand SE
DOI：
10.1016/j.spinee.2005.02.016
发表时间：
2005-11-01
期刊：
The spine journal : official journal of the North American Spine Society
影响因子：
--
作者：
Singh, Kern;Masuda, Koichi;An, Howard S
通讯作者：
An, Howard S
影响因子：
3
作者：
Chujo, Takehide;An, Howard S.;Masuda, Koichi
通讯作者：
Masuda, Koichi