Intervertebral Disc Degeneration Is Associated With Aberrant Endplate Remodeling and Reduced Small Molecule Transport.
Intervertebral Disc Degeneration Is Associated With Aberrant Endplate Remodeling and Reduced Small Molecule Transport.
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DOI：
10.1002/jbmr.4009
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发表时间：
2020-08
期刊：
影响因子：
--
通讯作者：
Gullbrand SE
中科院分区：
文献类型：
--
作者：
Ashinsky BG;Bonnevie ED;Mandalapu SA;Pickup S;Wang C;Han L;Mauck RL;Smith HE;Gullbrand SE
椎间盘是体内最大的无血管结构，椎间盘内的细胞依靠位于椎体终板内的血管系统进行扩散运输，以获得营养、清除废物和维持椎间盘健康。然而，小分子在体内进入椎间盘的机制以及这些参数如何随着椎间盘退变而变化的研究还不够深入。在这里，我们利用活体兔穿刺盘退变模型来研究这些相互作用，并提供证据表明，与椎间盘相邻的终板重塑伴随着退变发生。我们的结果发现终板骨体积分数显著增加，椎间盘和椎骨之间软组织界面的微尺度硬度增加，终板血管和小分子进入椎间盘的运输减少作为退变状态的函数。神经网络模型确定进入椎间盘的扩散变化是椎间盘退变的最重要的预测因素。这些发现支持了经终板运输在疾病进展中的关键作用，并将改善患者的选择，以指导适当的手术干预，并为改善椎间盘健康的新治疗方法提供信息。
The intervertebral disc is the largest avascular structure in the body, and cells within the disc rely on diffusive transport via vasculature located within the vertebral endplate to receive nutrients, eliminate waste products, and maintain disc health. However, the mechanisms by which small molecule transport into the disc occurs in vivo and how these parameters change with disc degeneration remain understudied. Here, we utilize an in vivo rabbit puncture disc degeneration model to study these interactions and provide evidence that remodeling of the endplate adjacent to the disc occurs concomitant with degeneration. Our results identify significant increases in endplate bone volume fraction, increases in microscale stiffness of the soft tissue interfaces between the disc and vertebral bone, and reductions in endplate vascularity and small molecule transport into the disc as a function of degenerative state. A neural network model identified changes in diffusion into the disc as the most significant predictor of disc degeneration. These findings support the critical role of trans-endplate transport in disease progression and will improve patient selection to direct appropriate surgical intervention and inform new therapeutic approaches to improve disc health.
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影响因子：
3
作者：
Benneker, LM;Heini, PF;Ito, K
通讯作者：
Ito, K
影响因子：
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作者：
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BAER, E
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影响因子：
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作者：
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