Microstructure and resident cell-types of the feline optic nerve head resemble that of humans.
Microstructure and resident cell-types of the feline optic nerve head resemble that of humans.
复制标题
猫科动物视神经头的微观结构和常驻细胞类型与人类相似。
DOI：
10.1016/j.exer.2020.108315
复制
发表时间：
2021-01
影响因子：
3.4
通讯作者：
McLellan GJ
中科院分区：
文献类型：
--
作者：
Oikawa K;Teixeira LBC;Keikhosravi A;Eliceiri KW;McLellan GJ
视神经乳头（ONH）的筛板（LC）区域被认为是青光眼损害的主要部位。在人类中，该区域的生物学反映了视网膜神经节细胞（RGC）轴突与其他驻留ONH细胞类型（包括星形胶质细胞、筛板细胞、小胶质细胞和少突胶质细胞）以及ONH微血管系统和胶原LC束之间的复杂相互作用。然而，该区域显微解剖学的物种差异可能会深刻影响在研究环境中建立青光眼病理生物学模型的努力。在这项研究中，我们的特点是居民细胞类型，ECM成分和超微结构的显微解剖的ONH在成年家猫（猫卡图斯）。用星形胶质细胞、小胶质细胞/巨噬细胞、少突胶质细胞、LC细胞和血管内皮细胞标记物对ONH组织的纵向和横向冷冻切片进行免疫标记。胶原纤维结构的LC可视化二次谐波产生（SHG）与多光子显微镜。纤维性星形胶质细胞在前层区域形成胶质细胞酸性蛋白（GFAP）阳性的胶质柱，并覆盖LC区域的胶原板，其方向垂直于轴突。GFAP阴性和α-平滑肌肌动蛋白阳性LC细胞被确定在猫ONH。IBA-1阳性免疫细胞和血管性血友病因子阳性血管内皮细胞也可在整个猫ONH中识别。在人类中，髓鞘形成开始于猫ONH的后板层区域中的少突胶质细胞群体。透射电子显微镜证实了毛细血管和LC细胞的存在，这些细胞在胶原LC梁的核心中延伸薄的过程。总之，猫ONH紧密地概括了人类和非人类灵长类动物ONH的复杂性，具有不同的ONH细胞类型和稳健的胶原LC，LC细胞和毛细血管驻留在胶原LC的束内。因此，在猫遗传性青光眼模型中的研究有可能在增强我们对青光眼视神经病变中ONH细胞和分子过程的理解中发挥关键作用。
The lamina cribrosa (LC) region of the optic nerve head (ONH) is considered a primary site for glaucomatous damage. In humans, biology of this region reflects complex interactions between retinal ganglion cell (RGC) axons and other resident ONH cell-types including astrocytes, lamina cribrosa cells, microglia and oligodendrocytes, as well as ONH microvasculature and collagenous LC beams. However, species differences in the microanatomy of this region could profoundly impact efforts to model glaucoma pathobiology in a research setting. In this study, we characterized resident cell-types, ECM composition and ultrastructure in relation to microanatomy of the ONH in adult domestic cats (Felis catus). Longitudinal and transverse cryosections of ONH tissues were immunolabeled with astrocyte, microglia/macrophage, oligodendrocyte, LC cell and vascular endothelial cell markers. Collagen fiber structure of the LC was visualized by second harmonic generation (SHG) with multiphoton microscopy. Fibrous astrocytes form glial fibrillary acidic protein (GFAP)-positive glial columns in the pre-laminar region, and cover the collagenous plates of the LC region in lamellae oriented perpendicular to the axons. GFAP-negative and alpha-smooth muscle actin-positive LC cells were identified in the feline ONH. IBA-1 positive immune cells and von Willebrand factor-positive blood vessel endothelial cells are also identifiable throughout the feline ONH. As in humans, myelination commences with a population of oligodendrocytes in the retro-laminar region of the feline ONH. Transmission Electron microscopy confirmed the presence of capillaries and LC cells that extend thin processes in the core of the collagenous LC beams. In conclusion, the feline ONH closely recapitulates the complexity of the ONH of humans and non-human primates, with diverse ONH cell-types and a robust collagenous LC, within the beams of which, LC cells and capillaries reside. Thus, studies in a feline inherited glaucoma model have the potential to play a key role in enhancing our understanding of ONH cellular and molecular processes in glaucomatous optic neuropathy.
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DOI：
10.1007/bf00448803
发表时间：
1996-09-01
影响因子：
2.7
作者：
Dichtl, A;Jonas, JB;Naumann, GOH
通讯作者：
Naumann, GOH
影响因子：
4.1
作者：
Albon, J;Karwatowski, WSS;Duance, VC
通讯作者：
Duance, VC
影响因子：
4.4
作者：
Brazile BL;Hua Y;Jan NJ;Wallace J;Gogola A;Sigal IA
通讯作者：
Sigal IA
影响因子：
2.5
作者：
Bosco, Alejandra;Steele, Michael R.;Vetter, Monica L.
通讯作者：
Vetter, Monica L.
影响因子：
3.4
作者：
HERNANDEZ, MR;YE, HQ;ROY, S
通讯作者：
ROY, S