Thin Lamina Cribrosa Beams Have Different Collagen Microstructure Than Thick Beams.
Thin Lamina Cribrosa Beams Have Different Collagen Microstructure Than Thick Beams.
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DOI：
10.1167/iovs.18-24763
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发表时间：
2018-09-04
影响因子：
4.4
通讯作者：
Sigal IA
中科院分区：
文献类型：
--
作者：
Brazile BL;Hua Y;Jan NJ;Wallace J;Gogola A;Sigal IA
比较薄筛板 (LC) 梁和厚筛板 (LC) 梁之间的胶原微结构卷曲特征。将四只羊的七只眼睛固定在 10% 福尔马林中，眼压为 5 mm Hg。对于每只眼睛，使用偏光显微镜对通过 LC 的一到三个冠状冷冻切片进行成像并进行分析，以可视化 LC 并确定胶原纤维微观结构。对于每根梁，我们测量了其宽度和胶原纤维卷曲的三个特征：波纹度、弯曲度和幅度。使用线性混合效应模型来测试压接特性是否与 LC 梁宽度相关。对于每只眼睛和所有眼睛，LC 光束宽度与卷曲波纹度和弯曲度呈正相关，与卷曲幅度呈负相关（P < 0.0001）。细梁的平均宽度为 13.11 μm，平均 (SD) 波纹度、弯曲度和振幅分别为 0.27 (0.17) 弧度、1.017 (0.028) 和 1.88 (1.41) μm。对于平均宽度为 26.10 μm 的厚梁，这些特性分别为 0.33 (0.18) 弧度、1.025 (0.037) 和 1.58 (1.36) μm。我们的结果表明液晶光束机械性能存在异质性。薄梁比厚梁的波动更小，这表明薄梁可能在比厚梁更低的眼压下变硬。这种差异可能允许细梁与较厚的梁一样支撑相似数量的 IOP 诱导力，从而在生理 IOP 下为轴突提供相似水平的结构支撑，尽管宽度存在差异。需要测量梁级机械性能来证实这些预测。
To compare the collagen microstructural crimp characteristics between thin and thick lamina cribrosa (LC) beams. Seven eyes from four sheep were fixed at 5 mm Hg IOP in 10% formalin. For each eye, one to three coronal cryosections through the LC were imaged with polarized light microscopy and analyzed to visualize the LC and determine collagen fiber microstructure. For every beam, we measured its width and three characteristics of the crimp of its collagen fibers: waviness, tortuosity, and amplitude. Linear mixed effects models were used to test whether crimp characteristics were associated with the LC beam width. For each eye and over all the eyes, LC beam width was positively associated with crimp waviness and tortuosity, and negatively associated with crimp amplitude (P's < 0.0001). Thin beams, average width 13.11 μm, had average (SD) waviness, tortuosity, and amplitude of 0.27 (0.17) radians, 1.017 (0.028) and 1.88 (1.41) μm, respectively. For thick beams, average width 26.10 μm, these characteristics were 0.33 (0.18) radians, 1.025 (0.037) and 1.58 (1.36) μm, respectively. Our results suggest heterogeneity in LC beam mechanical properties. Thin beams were less wavy than their thicker counterparts, suggesting that thin beams may stiffen at lower IOP than thick beams. This difference may allow thin beams to support similar amounts of IOP-induced force as thicker beams, thus providing a similar level of structural support to the axons at physiologic IOP, despite the differences in width. Measurements of beam-level mechanical properties are needed to confirm these predictions.
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人角膜和巩膜中胶原蛋白压接的空间模式和年龄相关的变化。
影响因子：
4.4
作者：
Gogola A;Jan NJ;Brazile B;Lam P;Lathrop KL;Chan KC;Sigal IA
通讯作者：
Sigal IA
影响因子：
3.4
作者：
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通讯作者：
Gerritsen HC
影响因子：
3.7
作者：
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通讯作者：
Sivak, Jeremy M.
影响因子：
3.5
作者：
Brown, Donald J.;Morishige, Naoyuki;Jester, James V.
通讯作者：
Jester, James V.
影响因子：
3.4
作者：
Burgoyne, Claude F.
通讯作者：
Burgoyne, Claude F.