Proteomic Profiling and Pathway Analysis of Acid Stress-Induced Vasorelaxation of Mesenteric Arteries In Vitro.
Proteomic Profiling and Pathway Analysis of Acid Stress-Induced Vasorelaxation of Mesenteric Arteries In Vitro.
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DOI：
10.3390/genes13050801
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发表时间：
2022-04-29
期刊：
影响因子：
3.5
通讯作者：
Mohanty, Bimal Prasanna
中科院分区：
文献类型：
--
作者：
Mohanty, Ipsita;Banerjee, Sudeshna;Mahanty, Arabinda;Mohanty, Sasmita;Nayak, Nihar Ranjan;Parija, Subas Chandra;Mohanty, Bimal Prasanna
关键词：
尽管代谢性酸中毒与许多病理生理条件有关，其血管舒张作用已在不同的动物和培养模型中得到很好的描述，但酸中毒诱导的血管舒张的分子机制尚未完全了解。肠系膜动脉模型已被广泛用于研究血管对各种病理生理条件的反应。我们之前的研究和其他一些报告表明，山羊肠系膜动脉和人类动脉对各种刺激（包括酸性应激）的血管反应非常相似。在这项研究中，为了进一步确定在酸性pH值下导致血管反应性改变的信号分子，我们使用山羊肠系膜动脉模型检测了酸胁迫诱导的血管松弛的蛋白质组学特征。采用2D-GE、7 cm IPG条带和微型凝胶、LC-MS/MS和MALDI TOF MS对酸性pH下的血管蛋白质组进行比较，质谱鉴定的独特蛋白为肌动蛋白、transgelin、WD重复蛋白1、desmin、原肌球蛋白、ATP合酶β、Hsp27、醛脱氢酶、丙酮酸激酶和维生素K环氧化物还原酶复合体亚基1样蛋白。在鉴定为肌动蛋白的5个蛋白点中，有3个上调了2倍。酸胁迫下ATP合酶β也上调了2.14倍。其他上调的肌动蛋白相关蛋白有transgelin、desmin和WD repeat-containing protein 1。等长收缩研究显示，酸中毒时，受体介导的（组胺）和非受体介导的（KCl）血管收缩减弱，而乙酰胆碱诱导的血管松弛增强。总的来说，在功能研究中观察到酸中毒时血管反应性的改变可能归因于肌动蛋白和ATP合成酶β表达的增加。
Although metabolic acidosis is associated with numerous pathophysiological conditions and its vasorelaxation effects have been well described in different animal and culture models, the molecular mechanisms of acidosis-induced vasorelaxation are not fully understood. Mesenteric artery models have been used extensively to examine the vascular response to various pathophysiological conditions. Our previous studies and several other reports have suggested the vascular responses of goat mesenteric arteries and human arteries to various stimuli, including acidic stress, are highly similar. In this study, to further identify the signaling molecules responsible for altered vasoreactivity in response to acidic pH, we examined the proteomic profile of acid stress-induced vasorelaxation using a goat mesenteric artery model. The vascular proteomes under acidic pH were compared using 2D-GE with 7 cm IPG strips and mini gels, LC-MS/MS, and MALDI TOF MS. The unique proteins identified by mass spectroscopy were actin, transgelin, WD repeat-containing protein 1, desmin, tropomyosin, ATP synthase β, Hsp27, aldehyde dehydrogenase, pyruvate kinase, and vitamin K epoxide reductase complex subunit 1-like protein. Out of five protein spots identified as actin, three were upregulated > 2-fold. ATP synthase β was also upregulated (2.14-fold) under acid stress. Other actin-associated proteins upregulated were transgelin, desmin, and WD repeat-containing protein 1. Isometric contraction studies revealed that both receptor-mediated (histamine) and non-receptor-mediated (KCl) vasocontraction were attenuated, whereas acetylcholine-induced vasorelaxation was augmented under acidosis. Overall, the altered vasoreactivity under acidosis observed in the functional studies could possibly be attributed to the increase in expression of actin and ATP synthase β.
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影响因子：
3.7
作者：
Capellini VK;Restini CB;Bendhack LM;Evora PR;Celotto AC
通讯作者：
Celotto AC
DOI：
10.1007/s43032-020-00402-4
发表时间：
2021-04
期刊：
Reproductive sciences (Thousand Oaks, Calif.)
影响因子：
--
作者：
Gokina NI;Fairchild RI;Bishop NM;Dawson TE;Prakash K;Bonney EA
通讯作者：
Bonney EA
影响因子：
1.6
作者：
Căpuşă C;Ştefan G;Stancu S;Lipan M;Tsur LD;Mircescu G
通讯作者：
Mircescu G
影响因子：
3.4
作者：
Bringans, Scott;Eriksen, Soren;Lipscombe, Richard
通讯作者：
Lipscombe, Richard
影响因子：
3.6
作者：
Nakanishi, T;Gu, H;Momma, K
通讯作者：
Momma, K