The antibody loci of the domestic goat (Capra hircus).
The antibody loci of the domestic goat (Capra hircus).
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DOI：
10.1007/s00251-017-1033-3
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发表时间：
2018-05
期刊：
影响因子：
3.2
通讯作者：
Hammond JA
中科院分区：
文献类型：
--
作者：
Schwartz JC;Philp RL;Bickhart DM;Smith TPL;Hammond JA
家山羊（Capra hircus）是一种重要的反刍动物物种，既可作为研究和生物医学应用中基于抗体的试剂的来源，又可作为重要的农业经济动物，特别是对于拥有全球大部分山羊种群的发展中国家而言。编码山羊免疫库的基因座的表征对于疫苗和免疫试剂的开发非常有益。然而，在使用短读长测序技术生成参考基因组的山羊和其他物种中，由于其重复性，免疫位点组装不良。我们最近构建的长读长山羊基因组组装 (ARS1) 有助于对所有三个抗体位点进行高置信度表征以及与牛的比较分析。我们观察到山羊和牛抗体编码位点的广泛相似性，但存在显着差异，可能影响功能性抗体库的形成。山羊重链基因座仅限于四个功能性且几乎相同的 IGHV 基因，而在牛中观察到的有 10 个。谱分析表明，山羊的轻链使用更加平衡，与牛 (~ 5%) 相比，kappa 轻链的代表性更高 (~ 20–30%)。本研究代表了山羊抗体位点的首次表征，并将有助于为未来研究其抗体对疾病和疫苗接种的反应提供信息。本文的在线版本 (10.1007/s00251-017-1033-3) 包含补充材料，可供授权用户使用。
The domestic goat (Capra hircus) is an important ruminant species both as a source of antibody-based reagents for research and biomedical applications and as an economically important animal for agriculture, particularly for developing nations that maintain most of the global goat population. Characterization of the loci encoding the goat immune repertoire would be highly beneficial for both vaccine and immune reagent development. However, in goat and other species whose reference genomes were generated using short-read sequencing technologies, the immune loci are poorly assembled as a result of their repetitive nature. Our recent construction of a long-read goat genome assembly (ARS1) has facilitated characterization of all three antibody loci with high confidence and comparative analysis to cattle. We observed broad similarity of goat and cattle antibody-encoding loci but with notable differences that likely influence formation of the functional antibody repertoire. The goat heavy-chain locus is restricted to only four functional and nearly identical IGHV genes, in contrast to the ten observed in cattle. Repertoire analysis indicates that light-chain usage is more balanced in goats, with greater representation of kappa light chains (~ 20–30%) compared to that in cattle (~ 5%). The present study represents the first characterization of the goat antibody loci and will help inform future investigations of their antibody responses to disease and vaccination. The online version of this article (10.1007/s00251-017-1033-3) contains supplementary material, which is available to authorized users.
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影响因子：
3
作者：
Ekman, Anna;Niku, Mikael;Iivanainen, Antti
通讯作者：
Iivanainen, Antti
DOI：
10.1093/bioinformatics/8.3.275
发表时间：
1992-06-01
期刊：
COMPUTER APPLICATIONS IN THE BIOSCIENCES
影响因子：
--
作者：
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通讯作者：
THORNTON, JM
影响因子：
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作者：
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通讯作者：
Uenishi, Hirohide
影响因子：
--
作者：
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通讯作者：
Lefranc, Marie-Paule
影响因子：
1.8
作者：
Butler, JE;Wertz, N;Wells, K
通讯作者：
Wells, K