High-fluence light emitting diode-red light inhibits cell cycle progression in human dermal fibroblasts.
High-fluence light emitting diode-red light inhibits cell cycle progression in human dermal fibroblasts.
复制标题
高通量发光二极管红光抑制人皮肤成纤维细胞的细胞周期进程。
DOI：
10.1002/jbio.202000359
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发表时间：
2021-03
影响因子：
2.8
通讯作者：
Jagdeo J
中科院分区：
文献类型：
--
作者：
Masub N;Austin E;Huang A;Jagdeo J
皮肤纤维化是几种全身性和皮肤病的衰弱特征。虽然目前的治疗方案存在很大的副作用和复发风险，但高通量发光二极管产生的红光(LED-RL)是一种安全、非侵入性和可获得的替代治疗方法。我们之前证明了LED-RL减少成纤维细胞的增殖，成纤维细胞增殖是纤维化的关键致病成分。然而，高通量LED-RL调控成纤维细胞增殖的细胞机制尚不清楚。在此，我们探讨了高通量LED-RL对人真皮成纤维细胞周期进程的影响。我们证明，与温度匹配的对照组相比，640J/cm2的LED-RL诱导G0/G1期细胞显著停滞。伴随而来的是受照细胞中检查点调节因子P53的表达相应增加。提示高能LED-RL可能通过诱导成纤维细胞G0/G1期停滞而发挥其抗增殖作用。此外，这项研究为LED-RL作为抗纤维化治疗的分子机制提供了洞察力。发光二极管红光(LED-RL)已被证明是一种潜在的皮肤纤维化治疗方法。我们先前报道LED-RL显著减少皮肤真皮成纤维细胞，而不诱导细胞凋亡。在此，我们证明了LED-RL能够抑制成纤维细胞的细胞周期进程，进一步阐明了LED-RL抑制成纤维细胞增殖的机制。
Skin fibrosis is a debilitating feature of several systemic and dermatologic diseases. While current treatment options carry significant risk of side effects and recurrence, high-fluence light emitting diode-generated red light (LED-RL) is an alternative therapeutic that is safe, non-invasive, and accessible. We previously demonstrated LED-RL decreases fibroblast proliferation, a key pathogenic component of fibrosis. However, the cellular mechanism by which high fluence LED-RL modulates fibroblast proliferation is unclear. Herein, we explored the effects of high fluence LED-RL on human dermal fibroblast cell cycle progression. We demonstrate that LED-RL at 640 J/cm2 induced significant arrest of cells in G0/G1 compared to temperature-matched control. This was accompanied by a corresponding increase in expression of checkpoint regulator p53 in irradiated cells. These data demonstrate high fluence LED-RL may exert its anti-proliferative effect on fibroblasts by inducing G0/G1 arrest. Further, this study provides insight into the molecular mechanism underlying LED-RL as an anti-fibrotic therapeutic. Light-emitting diode red light (LED-RL) has demonstrated promising evidence as a potential treatment for skin fibrosis. We previously reported LED-RL significantly decreased cutaneous dermal fibroblasts without inducing apoptosis. Herein, we demonstrate that LED-RL is capable of inhibiting cell cycle progression in fibroblasts, further elucidating the mechanism by which LED-RL induces its anti-proliferative effects on fibroblasts.
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影响因子：
2.9
作者：
Lichtman, Michael K.;Otero-Vinas, Marta;Falanga, Vincent
通讯作者：
Falanga, Vincent
发射二极管生成的红光调节与皮肤纤维化相关的特征。
影响因子：
2.8
作者：
Mamalis, Andrew;Koo, Eugene;Garcha, Manveer;Murphy, William J.;Isseroff, R. Rivkah;Jagdeo, Jared
通讯作者：
Jagdeo, Jared
血清饥饿诱导的细胞周期同步促进人类体细胞重编程
影响因子：
3.7
作者：
Chen, Mengfei;Huang, Jingjing;Ge, Jian
通讯作者：
Ge, Jian
影响因子：
7.2
作者：
Beckerman, Rachel;Prives, Carol
通讯作者：
Prives, Carol
影响因子：
6.2
作者：
Matsumura, Yasuhiro;Ananthaswamy, Honnavara N
通讯作者：
Ananthaswamy, Honnavara N