In vivo evidence for the unique kinetics of evoked dopamine release in the patch and matrix compartments of the striatum.
In vivo evidence for the unique kinetics of evoked dopamine release in the patch and matrix compartments of the striatum.
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DOI：
10.1007/s00216-021-03300-z
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发表时间：
2021-11
影响因子：
4.3
通讯作者：
Michael AC
中科院分区：
文献类型：
--
作者：
Jaquins-Gerstl A;Nesbitt KM;Michael AC
神经化学递质多巴胺（DA）与许多疾病状态有关，包括帕金森病、精神分裂症和药物滥用。大鼠脑背纹状体和伏隔核核心区的DA末端场呈异质结构域组织，表现出DA释放的快慢动力学。多巴胺的释放速度在快的区域明显比慢的区域快。纹状体由称为纹状体（斑块）和基质的空间隔室组成。关于斑块和基质隔室的空间组织及其功能，已有大量文献。然而，人们对这些区室知之甚少，因为它们与快速扫描循环伏安法（FSCV）观察到的快速和慢速动力学DA结构域有关。因此，我们将FSCV的高空间分辨率与使用荧光显微镜对这些建筑区室（斑块和基质）进行详细的免疫组织化学分析相结合。我们的研究结果表明，具有快速DA域动力学的斑块区室和具有缓慢DA域动力学的基质区室之间存在直接关联。我们还研究了纹状体中两个非常不同的亚区，外侧背纹状体（LDS）和内侧背纹状体（MDS）的动力学域。与内侧背纹状体相反，外侧背纹状体主要受快速动态DA域控制。这些发现是高度相关的，因为它们可能对揭示快速和慢速动力学DA结构域及其生理意义具有关键意义。在线版本包含补充材料，可在10.1007/s00216-021-03300-z获得。
The neurochemical transmitter dopamine (DA) is implicated in a number of diseases states, including Parkinson’s disease, schizophrenia, and drug abuse. DA terminal fields in the dorsal striatum and core region of the nucleus accumbens in the rat brain are organized as heterogeneous domains exhibiting fast and slow kinetic of DA release. The rates of dopamine release are significantly and substantially faster in the fast domains relative to the slow domains. The striatum is composed of a mosaic of spatial compartments known as the striosomes (patches) and the matrix. Extensive literature exists on the spatial organization of the patch and matrix compartments and their functions. However, little is known about these compartments as they relate to fast and slow kinetic DA domains observed by fast scan cyclic voltammetry (FSCV). Thus, we combined high spatial resolution of FSCV with detailed immunohistochemical analysis of these architectural compartments (patch and matrix) using fluorescence microscopy. Our findings demonstrated a direct correlation between patch compartments with fast domain DA kinetics and matrix compartments to slow domain DA kinetics. We also investigated the kinetic domains in two very distinct sub-regions in the striatum, the lateral dorsal striatum (LDS) and the medial dorsal striatum (MDS). The lateral dorsal striatum as opposed to the medial dorsal striatum is mainly governed by fast kinetic DA domains. These finding are highly relevant as they may hold key promise in unraveling the fast and slow kinetic DA domains and their physiological significance. The online version contains supplementary material available at 10.1007/s00216-021-03300-z.
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影响因子：
4.7
作者：
Bao, Li;Patel, Jyoti C.;Rice, Margaret E.
通讯作者：
Rice, Margaret E.
影响因子：
4.7
作者：
Wang Y;Moquin KF;Michael AC
通讯作者：
Michael AC
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作者：
Crittenden JR;Graybiel AM
通讯作者：
Graybiel AM
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作者：
Brimblecombe KR;Threlfell S;Dautan D;Kosillo P;Mena-Segovia J;Cragg SJ
通讯作者：
Cragg SJ
影响因子：
2.5
作者：
DI, S;BARTH, DS
通讯作者：
BARTH, DS