Cell-Free Spent Media Obtained from Bifidobacterium bifidum and Bifidobacterium crudilactis Grown in Media Supplemented with 3'-Sialyllactose Modulate Virulence Gene Expression in Escherichia coli O157:H7 and Salmonella Typhimurium.
Cell-Free Spent Media Obtained from Bifidobacterium bifidum and Bifidobacterium crudilactis Grown in Media Supplemented with 3'-Sialyllactose Modulate Virulence Gene Expression in Escherichia coli O157:H7 and Salmonella Typhimurium.
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DOI：
10.3389/fmicb.2016.01460
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发表时间：
2016
影响因子：
5.2
通讯作者：
Delcenserie V
中科院分区：
文献类型：
--
作者：
Bondue P;Crèvecoeur S;Brose F;Daube G;Seghaye MC;Griffiths MW;LaPointe G;Delcenserie V
人乳复合低聚糖（HMO）具有抑菌活性，能促进双歧杆菌如两歧双歧杆菌（Bifidobacterium bifidum）和长双歧杆菌（Bifidobacterium longum subsp.婴儿。此外，双歧杆菌对碳水化合物的发酵可导致产生对几种病原菌具有抗毒性作用的代谢物。乳清富含结构类似于HMO和B的复合牛乳低聚糖（BMO）。牛源的一个物种crudilactis能够代谢这些复合碳水化合物中的一些。本研究主要针对B.双歧杆菌B.在以3′-唾液酰乳糖（3′-saliyllactose，3′SL）为主要碳源的培养基中，粗乳杆菌（crudilactis）能够生长，而3 ′-saliyllactose是牛乳中的主要碳源。其次，测试了无细胞废培养基（CFSM）对大肠杆菌O 157：H7和沙门氏菌鼠伤寒血清型的毒力表达的影响。2株菌均能在3′SL存在下生长，但B.粗乳杆菌的生长最好（7.92 ± 0.3logcfu/ml）。双歧杆菌（6.84 ± 0.9对数cfu/ml）。然后，通过检测大肠杆菌发光突变体的ler和hilA启动子活性，检测CFSM对毒力基因表达的影响。coli和革兰氏阳性菌S.鼠伤寒沙门氏菌和野生型菌株。coli O 157：H7和S.使用RT-qPCR检测鼠伤寒沙门氏菌。所有的CFSM都导致发光突变株的ler和hilA基因以及野生型大肠杆菌菌株的ler（比例分别为−15.4和−8.1）和qseA（比例分别为−2.1和−3.1）的显著表达不足。大肠杆菌O 157：H7。因此，3′SL（一种主要的BMO）与一些牛源或人源双歧杆菌菌株组合可能是一种有趣的合生元，用于维持或恢复幼儿的肠道健康。将进一步研究在体外观察到的这些作用，以了解病原体的总体表型和活性分子的确切性质。
Complex oligosaccharides from human milk (HMO) possess an antimicrobial activity and can promote the growth of bifidobacteria such as Bifidobacterium bifidum and Bifidobacterium longum subsp. infantis. In addition, fermentation of carbohydrates by bifidobacteria can result in the production of metabolites presenting an antivirulence effect on several pathogenic bacteria. Whey is rich in complex bovine milk oligosaccharides (BMO) structurally similar to HMO and B. crudilactis, a species of bovine origin, is able to metabolize some of those complex carbohydrates. This study focused on the ability of B. bifidum and B. crudilactis to grow in a culture medium supplemented in 3′-sialyllactose (3′SL) as the main source of carbon, a major BMO encountered in cow milk. Next, the effects of cell-free spent media (CFSM) were tested against virulence expression of Escherichia coli O157:H7 and Salmonella enterica serovar Typhimurium. Both strains were able to grow in presence of 3′SL, but B. crudilactis showed the best growth (7.92 ± 0.3 log cfu/ml) compared to B. bifidum (6.84 ± 0.9 log cfu/ml). Then, CFSM were tested for their effects on virulence gene expression by ler and hilA promoter activity of luminescent mutants of E. coli and S. Typhimurium, respectively, and on wild type strains of E. coli O157:H7 and S. Typhimurium using RT-qPCR. All CFSM resulted in significant under expression of the ler and hilA genes for the luminescent mutants and ler (ratios of −15.4 and −8.1 respectively) and qseA (ratios of −2.1 and −3.1) for the wild type strain of E. coli O157:H7. The 3′SL, a major BMO, combined with some bifidobacteria strains of bovine or human origin could therefore be an interesting synbiotic to maintain or restore the intestinal health of young children. These effects observed in vitro will be further investigated regarding the overall phenotype of pathogenic agents and the exact nature of the active molecules.
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双歧杆菌和碳水化合物代谢的基因组。
影响因子：
--
作者：
Bondue P;Delcenserie V
通讯作者：
Delcenserie V
影响因子：
7.3
作者：
Arrieta MC;Stiemsma LT;Amenyogbe N;Brown EM;Finlay B
通讯作者：
Finlay B
影响因子：
4.2
作者：
Delcenserie V;Taminiau B;Gavini F;de Schaetzen MA;Cleenwerck I;Theves M;Mahieu M;Daube G
通讯作者：
Daube G
影响因子：
2.2
作者：
Carey, Christine M.;Kostrzynska, Magdalena;Thompson, Stacey
通讯作者：
Thompson, Stacey
影响因子：
3.1
作者：
Barile D;Tao N;Lebrilla CB;Coisson JD;Arlorio M;German JB
通讯作者：
German JB