High-resolution imaging of the mouse-hair-cell hair bundle by scanning electron microscopy.
High-resolution imaging of the mouse-hair-cell hair bundle by scanning electron microscopy.
复制标题
扫描电子显微镜对小鼠毛细胞毛束的高分辨率成像。
DOI：
10.1016/j.xpro.2022.101213
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发表时间：
2022-03-18
期刊：
影响因子：
--
通讯作者：
Grillet N
中科院分区：
文献类型：
--
作者：
Grillet N
扫描电子显微镜（SEM）允许以亚纳米分辨率对细胞表面成像。然而，样品需要特定的制备以维持SEM的高真空并具有导电性。样本制备包括解剖、固定、脱水、金属涂层和组织封固。在这里，我们提供了一个全面的协议，执行扫描电镜对小鼠的内耳，并在高分辨率的毛束图像。毛束是位于毛细胞顶面的力敏感细胞器。有关本方案使用和执行的完整详细信息，请参阅。小鼠内耳感觉上皮的组织学和解剖毛细胞扫描电子显微镜的样品制备允许机械转导细胞器和尖端链接的成像扫描电子显微镜（SEM）允许以亚纳米分辨率对细胞表面成像。然而，样品需要特定的制备以维持SEM的高真空并具有导电性。样本制备包括解剖、固定、脱水、金属涂层和组织封固。在这里，我们提供了一个全面的协议，执行扫描电镜对小鼠的内耳，并在高分辨率的毛束图像。毛束是位于毛细胞顶面的力敏感细胞器。
Scanning electron microscopy (SEM) allows cell surface imaging at a sub-nanometric resolution. However, the sample requires a specific preparation to sustain the high vacuum of the SEM and be electrically conductive. The sample preparation consists of dissection, fixation, dehydration, metal coating, and tissue mounting. Here we provide a comprehensive protocol to perform SEM on the mouse’s inner ear, and image the hair bundles at high resolution. Hair bundles are the force-sensitive organelles located at the apical surface of hair cells. For complete details on the use and execution of this protocol, please refer to. Histology and dissection of the mouse’s inner ear sensory epithelium Sample preparation for scanning electron microscopy of the hair cells Allows imaging of the mechanotransduction organelle and the tip links Scanning electron microscopy (SEM) allows cell surface imaging at a sub-nanometric resolution. However, the sample requires a specific preparation to sustain the high vacuum of the SEM and be electrically conductive. The sample preparation consists of dissection, fixation, dehydration, metal coating, and tissue mounting. Here we provide a comprehensive protocol to perform SEM on the mouse’s inner ear, and image the hair bundles at high resolution. Hair bundles are the force-sensitive organelles located at the apical surface of hair cells.
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影响因子：
5.5
作者：
Miller KK;Atkinson P;Mendoza KR;Ó Maoiléidigh D;Grillet N
通讯作者：
Grillet N
影响因子：
3.3
作者：
Furness, D. N.;Katori, Y.;Hackney, C. M.
通讯作者：
Hackney, C. M.
影响因子：
2.8
作者：
FURNESS, DN;HACKNEY, CM
通讯作者：
HACKNEY, CM
DOI：
10.1523/jneurosci.0975-20.2021
发表时间：
2021-04-14
期刊：
The Journal of neuroscience : the official journal of the Society for Neuroscience
影响因子：
--
作者：
Trouillet A;Miller KK;George SS;Wang P;Ali NE;Ricci A;Grillet N
通讯作者：
Grillet N
影响因子：
1.4
作者：
HEYWOOD, P;RESNICK, S
通讯作者：
RESNICK, S