Local Differences in the Toxin Amount and Composition of Tetrodotoxin and Related Compounds in Pufferfish (Chelonodon patoca) and Toxic Goby (Yongeichthys criniger) Juveniles.
Local Differences in the Toxin Amount and Composition of Tetrodotoxin and Related Compounds in Pufferfish (Chelonodon patoca) and Toxic Goby (Yongeichthys criniger) Juveniles.
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DOI：
10.3390/toxins14020150
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发表时间：
2022-02-18
期刊：
影响因子：
4.2
通讯作者：
Itoi S
中科院分区：
文献类型：
--
作者：
Ito M;Furukawa R;Yasukawa S;Sato M;Oyama H;Okabe T;Suo R;Sugita H;Takatani T;Arakawa O;Adachi M;Nishikawa T;Itoi S
携带河豚毒素（TTX）的鱼类通过食物链从猎物中摄取TTX，并在体内积累TTX。虽然已经报道了各种各样的携带TTX的生物体，但TTX供应链中缺失的环节尚未完全阐明。在这里，我们调查了河豚鱼，Chelonodon patoca，和有毒的虾虎鱼，Yongeichthys criniger，使用LC-MS/MS的青少年的TTX和5，6，11-trideoxyTTX的组合物，以解决TTX供应链中缺失的环节。不同地点、不同采样期和不同鱼种的样品中TTX浓度存在差异。在同一地点的样品中，TTX浓度显着高于有毒的虾虎鱼幼鱼比在河豚鱼幼鱼。河豚鱼幼鱼体内TTX浓度显著高于5，6，11-三脱氧TTX，而虾虎鱼体内TTX和5，6，11-三脱氧TTX的组成比例在不同采样点存在差异。然而，从同一地点不同时期收集的虾虎鱼样本中TTX/5，6，11-三脱氧TTX比例没有差异。基于种特异性PCR，有毒的扁形虫（Planocera multitentaculata）特异性序列（细胞色素c氧化酶亚基I）的检测率也不同的河豚和有毒虾虎鱼的肠道内容物收集在不同的地点和时期。这些结果表明，虽然有毒扁形虫的幼虫可能是负责河豚鱼和有毒虾虎鱼的青少年的河豚毒素，这些鱼的青少年也可能会根据其栖息地的其他河豚毒素的生物饲料，他们也有不同的积累机制的河豚毒素和5，6，11-三脱氧河豚毒素。
Tetrodotoxin (TTX)-bearing fish ingest TTX from their preys through the food chain and accumulate TTX in their bodies. Although a wide variety of TTX-bearing organisms have been reported, the missing link in the TTX supply chain has not been elucidated completely. Here, we investigated the composition of TTX and 5,6,11-trideoxyTTX in juveniles of the pufferfish, Chelonodon patoca, and toxic goby, Yongeichthys criniger, using LC–MS/MS, to resolve the missing link in the TTX supply chain. The TTX concentration varied among samples from different localities, sampling periods and fish species. In the samples from the same locality, the TTX concentration was significantly higher in the toxic goby juveniles than in the pufferfish juveniles. The concentration of TTX in all the pufferfish juveniles was significantly higher than that of 5,6,11-trideoxyTTX, whereas the compositional ratio of TTX and 5,6,11-trideoxyTTX in the goby was different among sampling localities. However, the TTX/5,6,11-trideoxyTTX ratio in the goby was not different among samples collected from the same locality at different periods. Based on a species-specific PCR, the detection rate of the toxic flatworm (Planocera multitentaculata)-specific sequence (cytochrome c oxidase subunit I) also varied between the intestinal contents of the pufferfish and toxic goby collected at different localities and periods. These results suggest that although the larvae of the toxic flatworm are likely to be responsible for the toxification of the pufferfish and toxic goby juveniles by TTX, these fish juveniles are also likely to feed on other TTX-bearing organisms depending on their habitat, and they also possess different accumulation mechanisms of TTX and 5,6,11-trideoxyTTX.
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在饮食中包括planocerid flat虫可以有效地诱捕pufferfish，takifugu niphobles。
影响因子：
4.6
作者：
Itoi S;Ueda H;Yamada R;Takei M;Sato T;Oshikiri S;Wajima Y;Ogata R;Oyama H;Shitto T;Okuhara K;Tsunashima T;Sawayama E;Sugita H
通讯作者：
Sugita H
影响因子：
2.1
作者：
GOTO, T;KISHI, Y;HIRATA, Y
通讯作者：
HIRATA, Y
影响因子：
2.8
作者：
Gall, Brian G.;Stokes, Amber N.;Brodie, Edmund D., Jr.
通讯作者：
Brodie, Edmund D., Jr.
影响因子：
10.7
作者：
Katoh K;Standley DM
通讯作者：
Standley DM
影响因子：
56.9
作者：
MOSHER, HS;FISCHER, HG;BUCHWALD, HD
通讯作者：
BUCHWALD, HD